RT journal article T1 Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures A1 Bravo Porras, Gloria A1 Fernández Güelfo, Luis Alberto A1 Álvarez Gallego, Carlos José A1 Carbú Espinosa de los Monteros, María A1 Sales Márquez, Diego A1 Romero García, Luis Isidoro A2 BiomedicinaBiotecnología y Salud Pública A2 Ingeniería Química y Tecnología de Alimentos A2 Tecnologías del Medio Ambiente K1 Polyhydroxyalkanoates K1 Bioplastic K1 Volatile fatty acids K1 Mixed microbial cultures AB Polyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potentialcheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluentfrom dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine its capability toproduce PHAs from nine different synthetic mixtures of volatile fatty acids (VFAs). The tests consisted of mixtures of acetic,propionic, butyric, and valeric acids in the range of 1.5–9.0 g/L of total acidity and with three different valeric:butyric ratios(10:1, 1:1, and 1:10). Experimental results have shown a consistent preference of the MMC for the butyric and valeric acidsas carbon source instead other shorter acids (propionic or acetic) in terms of PHA production yield (estimated in dry cellweight basis), with a maximum value of 23% w/w. Additionally, valeric-rich mixtures have demonstrated to carry out a fastdegradation process but with poor final PHA production compared with high butyric mixtures. Finally, high initial butyricand valeric concentrations (1.1 g/L and 4.1 g/L) have demonstrated to be counterproductive to PHA production. PB SPRINGER HEIDELBERG SN 2190-6815 YR 2021 FD 2021-12 LK http://hdl.handle.net/10498/25935 UL http://hdl.handle.net/10498/25935 LA eng NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was supported by the project CTM2016-79071-R (Spanish Ministry of Economy, Industry and Competitiveness) and financed by the Spanish State Research Agency ("Agencia Estatal de Investigacion" (AEI)) and by the European Regional Development Fund (ERDF) and by grant from the Program for the Promotion and Impulse of Research and Transfer of the University of Cadiz (Ref: IRTP04_UCA). DS Repositorio Institucional de la Universidad de Cádiz RD 21-sep-2026