RT journal article T1 Evaluation of Microbial Communities of Bottled Mineral Waters and Preliminary Traceability Analysis Using NGS Microbial Fingerprints A1 Carraturo, Federica A1 Del Giudice, Carmela A1 Compagnone, María Cristina A1 Compagnone, María Cristina A1 Libralato, Giovanni A1 Toscanesi, Maria A1 Trifuoggi, Marco A1 Galdiero, Emilia A1 Guida, Marco A2 Ingeniería Química y Tecnología de Alimentos K1 mineral water K1 shelf life K1 NGS K1 microbiota K1 fingerprints K1 traceability AB The microbiological monitoring of mineral bottled waters results is crucial for the preventionof outbreaks in consumers. European and International regulations establish the quality ofwater intended for human consumption in order to preserve human health from the negative effectsderiving from water contamination. Advanced methods targeting the faster detection of potentialpathogens in drinking water may consent to the creation of an early warning system, enhancingwater quality management. This study aimed to suggest the implementation of standard waterquality evaluations, based on the characterization of the microbial composition of mineral bottledwater brands, contributing to the periodic control of the water’s microbiological stability along withthe shelf life, and, consequently, the stability of the supplying sources. Bottled water microbiotaanalysis was combined with the qualitative and quantitative evaluation of microbial loads in time,and the monitoring was performed in two seasons and two different storage conditions for a total ofsixty days. The employment of molecular microbiology techniques (NGS and Sanger sequencing),compared to standardized cultural methods and integrated with metagenomic analysis, combiningchemical and physical indicators for each sample, allowing for the generation of specific fingerprintsfor mineral bottled waters, pointing at simplifying and improving the foreseen risk assessmentstrategies to ensure the adequate traceability, quality and safety management of drinking water. PB MDPI SN 2073-4441 YR 2021 FD 2021-10 LK http://hdl.handle.net/10498/25956 UL http://hdl.handle.net/10498/25956 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 22-sep-2026