RT journal article
T1 Effect of Several Pretreatments on the Lactic Acid Production from Exhausted Sugar Beet Pulp
A1 Marzo Gago, Cristina
A1 Díaz Sánchez, Ana Belén
A1 Caro Pina, Ildefonso
A1 Blandino Garrido, Ana María
A2 Ingeniería Química y Tecnología de Alimentos
K1 sugar beet pulp
K1 biological pretreatment
K1 alkaline hydrogen peroxide pretreatment
K1 thermochemical pretreatment
K1 enzymatic hydrolysis
K1 lactic acid fermentation
AB Exhausted sugar beet pulp (ESBP), a by-product of the sugar industry, has been used as a substrate to produce lactic acid (LA). Due to the fact that ESBP contains a high percentage of pectin and hemicellulose, different pretreatments were studied to solubilize them and to facilitate the access to cellulose in the subsequent enzymatic hydrolysis. Several pretreatments were studied, specifically biological, oxidant with alkaline hydrogen peroxide (AHP), and thermochemical with acid (0.25, 0.5, or 1% w/v of H2SO4). Pretreated ESBP was enzymatically hydrolysed and fermented with the strain Lactiplantibacillus plantarum for LA production. The hydrolysis was carried out with the commercial enzymes Celluclast, pectinase, and xylanase, for 48 h. After that, the hydrolysate was supplemented with yeast extract and calcium carbonate before the bacteria inoculation. Results showed that all the pretreatments caused a modification of the fibre composition of ESBP. In most cases, the cellulose content increased, rising from 25% to 68% when ESBP was pretreated thermochemically at 1% w/v H2SO4. The production of LA was enhanced when ESBP was pretreated thermochemically. However, it was reduced when biological and AHP pretreatments were applied. In conclusion, thermochemical pretreatment with 1% w/v H2SO4 had a positive impact on the production of LA, increasing its concentration from 27 g/L to 50 g/L.
PB MDPI
SN 2304-8158
YR 2021
FD 2021-10
LK http://hdl.handle.net/10498/26012
UL http://hdl.handle.net/10498/26012
LA eng
NO This research was funded by "Ministerio de Economia, Industria y Competitividad", "Agencia Estatal de Investigacion (AEI)" and "Fondo Europeo de Desarrollo Regional (FEDER)" (CTM2016-79071-R).
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026