RT journal article
T1 UV-Vis Spectrophotometry and UPLC-PDA Combined with Multivariate Calibration for Kappaphycus alvarezii (Doty) Doty ex Silva Standardization Based on Phenolic Compounds
A1 Mutiarahma, Selma
A1 Putra, Venansius G. P.
A1 Chaniago, Weni
A1 Carrera Fernández, Ceferino Adrián
A1 Anggrahini, Sri
A1 Palma Lovillo, Miguel
A1 Setyaningsih, Widiastuti
A2 Química Analítica
K1 chemometrics
K1 flavonoid
K1 partial least squares
K1 phenolic acid
K1 principal component analysis
AB The algae Kappaphycus alvarezii is considered an important raw material for industrial practices, producing high economic value of various derived products. However, the quality of this commodity, which can be indicated by the level of phenolic compounds, may vary due to growth factors, including cultivation sites. An analytical UV-Vis spectrophotometry method coupled with chemometrics was proposed to standardize the red alga based on the content of phenolic compounds. The correlation between the UV-Vis spectra and UPLC-PDA results, combined with a multivariate calibration of the K. alvarezii extracts, was analyzed. The extracts were prepared using an ultrasound-based technique and subsequently subjected to UV-Vis spectral measurements at 200-800 nm and UPLC-PDA at 260 and 330 nm. Chemometric techniques and partial least squares (PLS) were applied to the acquired data to build a reliable analysis of the phenolics in the K. alvarezii extracts. The result showed that the wavelength combination of 200-450 and 600-690 nm provided a valid method for quantitative analysis of the studied phenolics that belong to hydroxybenzoic acid, hydroxycinnamic acid, and flavonoid with a coefficient of regression (R-2) > 0.96 in the calibration and validation models, along with an RMSEC and RMSEP value < 8%. The method was then employed to characterize the K. alvarezii samples from 13 different cultivation areas. Principal component analysis (PCA) generated principal components that produced a clear distribution among the samples of K. alvarezii based on phenolic compounds corresponding to the geographical origin.
PB MDPI
SN 2218-0532
YR 2021
FD 2021
LK http://hdl.handle.net/10498/26423
UL http://hdl.handle.net/10498/26423
LA eng
NO This research forms part of the National Research Priority (PRN) project on the standardization of Indonesian algae funded by the BOPTN Research Grant 006/E4.1/AK.04.PRN/2021 from the Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia. The APC was supported by Universitas Gadjah Mada through the Final Project Recognition Program (Rekognisi Tugas Akhir, RTA) 2020 with assignment number 3143/UN1.P.III/DIT-LIT/PT/2021.
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026