RT journal article
T1 An ultrasound-based technique for the analytical extraction of phenolic compounds in red algae
A1 Putra, Venansius G. P.
A1 Mutiarahma, Selma
A1 Chaniago, Weni
A1 Rahmadi, Puji
A1 Kurnianto, Dedy
A1 Hidayat, Chusnul
A1 Carrera Fernández, Ceferino Adrián
A1 Palma Lovillo, Miguel
A1 Setyaningsih, Widiastuti
A2 Química Analítica
K1 Rhodophyta
K1 Optimization
K1 Box-Behnken design
K1 Response surface methodology
K1 Method validation
AB Phenolic compounds are bioactive compounds that are also naturally found in red algae. To determine the level of these compounds in the red algae, spectroscopic or chromatographic determination was applied over the liquid extracts. Therefore, a prior extraction method is needed. The presented study aimed to develop the analytical ultrasound-assisted extraction (UAE) method to extract phenolic compounds from red algae. A Box-Behnken design (BBD) based on five factors included solvent composition (50-90% ethanol in water), extraction temperature (10-60 degrees C), ultrasonic power (20-100%), pulse duty-cycle (0.2-1.0 s(-1)), and solvent-to-sample ratio (10:1 to 30:1) was used to evaluate the effects of the studied factors. Subsequently, response surface methodology (RSM) was performed to define the optimum extraction condition to recover phenolic compounds from the alga matrices. The UAE condition suggested by RSM was: ultrasonic power 100%, pulse duty-cycle 1 s(-1), temperature 52.5 degrees C, extraction solvent 50% ethanol in water, and solvent-to-sample ratio 30:1. Kinetic studies confirmed 10 min to provide comparable recovery (p > 0.05) than any longer extraction time. The acceptable values validated the developed method for repeatability (CV, 4.8%) and intermediate precision (CV, 5.7%). In addition, the accuracy of the method suggested a complete recovery for two extraction cycles. Furthermore, the method has successfully been applied for a number of samples covering three different red algae species. Fingerprints of each sample based on phenolic composition and levels characterize the type and origin of different red algae species. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
PB ELSEVIER
SN 1878-5352
YR 2022
FD 2022-02
LK http://hdl.handle.net/10498/26429
UL http://hdl.handle.net/10498/26429
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026