RT journal article
T1 Dysregulation of Intestinal Physiology by Aflatoxicosis in the Gilthead Seabream (Sparus aurata)
A1 Barany Ruiz, Andre
A1 Oliva Ramírez, Milagrosa
A1 Filipa Gregório, Silvia
A1 Martínez-Rodríguez, Gonzalo
A1 Mancera Romero, Juan Miguel
A1 Fuentes, Juan
A2 Biología
K1 epithelial barrier
K1 gastrointestinal tract
K1 mycotoxin
K1 tight junctions
K1 Ussing chamber
AB Aflatoxin B1 (AFB1) is a mycotoxin often present in food. This study aimed to understand the physiological effects of AFB1 on the seabream (Sparus aurata) gastrointestinal system. In a first in vitro approach, we investigated ion transport using the short-circuit current (Isc) technique in Ussing chambers in the anterior intestine (AI). Application of apical/luminal AFB1 concentrations of 8 and 16 mu M to healthy tissues was without effect on tissue transepithelial electrical resistance (TER), and apparent tissue permeability (Papp) was measured using fluorescein FITC (4 kD). However, it resulted in dose-related effects on Isc. In a second approach, seabream juveniles fed with different AFB1 concentrations (1 and 2 mg AFB1 kg(-1) fish feed) for 85 days showed significantly reduced gill Na+/K+-ATPase (NKA) and H+-ATPase (HA) activities in the posterior intestine (PI). Moreover, dietary AFB1 modified Isc in the AI and PI, significantly affecting TER in the AI. To understand this effect on TER, we analyzed the expression of nine claudins and three occludins as markers of intestinal architecture and permeability using qPCR. Around 80% of the genes presented significantly different relative mRNA expression between AI and PI and had concomitant sensitivity to dietary AFB1. Based on the results of our in vitro, in vivo, and molecular approaches, we conclude that the effects of dietary AFB1 in the gastrointestinal system are at the base of the previously reported growth impairment caused by AFB1 in fish.
PB FRONTIERS MEDIA SA
SN 1664-042X
YR 2021
FD 2021-12
LK http://hdl.handle.net/10498/26437
UL http://hdl.handle.net/10498/26437
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026