RT journal article
T1 A novel magnetic molecularly imprinted polymer for selective extraction and determination of quercetin in plant samples
A1 Karrat, Abdelhafid
A1 Palacios Santander, José María
A1 Amine, Aziz
A1 Cubillana Aguilera, Laura
A2 Química Analítica
K1 Molecularly imprinted polymers
K1 Solid-phase extraction
K1 Magnetic nanoparticles
K1 Quercetin
K1 Fluorescence detection
K1 Paper-based analytical device
K1 High energy ultrasound-based synthesis
AB Molecularly imprinted polymers that mimic the binding mechanism of antibodies and their antigensexhibit several advantages, such as fast synthesis, low cost, high stability, and allow to overcome theethical issues associated with antibody farming in animals. Herein, a novel strategy combining themagnetic molecularly imprinted polymer (MMIP) as an artificial antibody with a fluorescence procedurefor the detection of quercetin in plant samples was designed. The MMIP was synthesized via a radicalpolymerization process to recognize specific functional groups of quercetin using a green techniquebased on high energy ultrasound irradiation. The developed MMIP was characterized by Fourier transforminfrared spectroscopy, X-ray diffraction, scanning/transmission electron microscopy, and thermalgravimetric analysis, which confirmed the successful preparation of MMIP. The adsorption capacity andselectivity of the MMIP for quercetin and other interferents analogous were performed. The MMIP wasapplied in the solid-phase extraction (SPE) technique as a selective sorbent for the sample preparation.Besides, a sensitive fluorometric method for the quantitation of quercetin was developed. A linearresponse was obtained within the concentration of 0.005e1.25 mg mL 1 of quercetin. The limit ofdetection and quantitation were 1.1 ng mL 1 and 3.7 ng mL 1, respectively. The average recoveries forquercetin were between 92.2% and 104.7% with an RSD less than 5.06% in spiked orange juice and teaextract samples. Furthermore, the developed procedure was successfully combined with a new paperbasedanalytical device for on-site smartphone analysis of quercetin.© 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-NDlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/).
PB ELSEVIER
SN 0003-2670
YR 2022
FD 2022-04
LK http://hdl.handle.net/10498/26492
UL http://hdl.handle.net/10498/26492
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026