RT journal article
T1 Unravelling the Initial Triggers of Botrytis cinerea Infection: First Description of Its Surfactome
A1 Escobar Niño, Almudena
A1 Carrasco Reinado, Rafael
A1 Morano Bermejo, Inés M.
A1 Cantoral Fernández, Jesús Manuel
A1 Fernández Acero, Francisco Javier
A2 BiomedicinaBiotecnología y Salud Pública
K1 Botrytis cinerea
K1 fungal phytopathogen
K1 surfactome
K1 proteomics
K1 signaling cascades
AB Botrytis cinerea is a critically important phytopathogenic fungus, causing devastating crop losses; signal transduction cascades mediate the "dialogue" among the fungus, plant, and environment. Surface proteins play important roles as front-line receptors. We report the first description of the surfactome of a filamentous fungus. To obtain a complete view of these cascades during infection of B. cinerea, its surfactome has been described by optimization of the "shaving" process and LC-MS/MS at two different infection stages, and with both rapid and late responses to environmental changes. The best results were obtained using PBS buffer in the "shaving" protocol. The surfactome obtained comprises 1010 identified proteins. These have been categorized by gene ontology and protein-protein interactions to reveal new potential pathogenicity/virulence factors. From these data, the percentage of total proteins predicted for the genome of the fungus represented by proteins identified in this and other proteomics studies is calculated at 54%, a big increase over the previous 12%. The new data may be crucial for understanding better its biological activity and pathogenicity. Given its extensive exposure to plants and environmental conditions, the surfactome presents innumerable opportunities for interactions between the fungus and external elements, which should offer the best targets for fungicide development.
PB MDPI
SN 2309-608X
YR 2021
FD 2021-12
LK http://hdl.handle.net/10498/26610
UL http://hdl.handle.net/10498/26610
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026