RT journal article
T1 Functional and Molecular Immune Response of Rainbow Trout (Oncorhynchus mykiss) Following Challenge with Yersinia ruckeri
A1 Fajardo Quiñones, Carlos
A1 Santos, Paulo
A1 Passos, Ricardo
A1 Vaz, Mariana
A1 Azeredo, Rita
A1 Machado, Marina
A1 Fernandez-Boo, Sergio
A1 Baptista, Teresa
A1 Costas, Benjamín
A2 BiomedicinaBiotecnología y Salud Pública
K1 biomarkers
K1 ERM
K1 hematological profile
K1 oxidative stress
K1 yersiniosis
AB Currently, aquaculture production of rainbow trout (Oncorhynchus mykiss) is a multibillion dollar industry; nevertheless, the development of this sector has not been exempt from pitfalls related to the recurrent presence of pathogens of bacterial origin. This is the case of Yersinia ruckeri, the etiologic agent of the infectious pathology known as Enteric Red Mouth Disease (ERM), causing serious economic losses that can be as high as 30-70% of production. Although several studies have been performed regarding pathogen features and virulence factors, more information is needed about the host defense mechanism activation after infection. Given this perspective, this study aimed to evaluate rainbow trout's short-term innate immune response against infection with Y. ruckeri. A series of factors linked to the innate immune response were evaluated, including determination of hematological parameters, oxidative stress biomarkers, and analysis of the expression of immune-related genes. Results showed a significant decrease in several hematological parameters (white blood cell count, hematocrit, neutrophils, monocytes, lymphocytes, and thrombocytes) and oxidative stress indicators (SOD) between the control and infected groups. In addition, there were significant differences in the level of gene expression between infected individuals and the control group. Most of these genes (il-1 beta, il-8, il-10, tnf-alpha 1, tnf-alpha 2, socs3, mmp-9, cath, hsp-70, saa, fer, pcb) were upregulated within the first 24 h following infection. Results from this study showed more insights into the short-term immune response of rainbow trout to infection with Y. ruckeri, which may be useful for the establishment of biomarkers that may be used for the early detection of ERM.
PB MDPI
SN 1422-0067
YR 2022
FD 2022-03
LK http://hdl.handle.net/10498/26742
UL http://hdl.handle.net/10498/26742
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026