RT journal article T1 A comparison study between ultrasound-assisted and enzyme-assisted extraction of anthocyanins from blackcurrant (Ribes nigrum L.) A1 Aliaño González, María José A1 Carrera Fernández, Ceferino Adrián A1 Fernández Barbero, Gerardo A1 Palma Lovillo, Miguel A2 Química Analítica K1 Anthocyanins K1 Ultrasound–assisted extraction K1 Enzyme–assisted extraction K1 Blackcurrant K1 Ribes nigrum K1 Plackett–Burman K1 Box-Behnken K1 Comparison AB Blackcurrant (Ribes nigrum L.) is a fruit rich in vitamins, fatty acids, minerals, essential oils and phenolic com-pounds, including anthocyanins. In the present work, two anthocyanin extraction methods from blackcurrant samples based on Ultrasound-Assisted Extraction (UAE) and Enzyme-Assisted Extraction (EAE) have been developed. A Plackett-Burman design with seven variables has been preliminary used for both UAE and EAE in order to determine the most influential variables in each methodology. After that, a Box-Behnken design was employed to optimize the extraction methods. The composition of the extraction solvent (% EtOH in water) has been the most influential variable for both UAE and EAE. The optimal extraction times have been 5 min for UAE and 10 min for EAE. No differences have been observed in anthocyanin extraction with both methodologies. Both methods have been applied to blackcurrant-derived products and proven their suitability for quality control analysis. PB ELSEVIER SN 2590-1575 YR 2022 FD 2022-03 LK http://hdl.handle.net/10498/26937 UL http://hdl.handle.net/10498/26937 LA eng NO This work has been supported by the project "EQC2018-005135-P" (Equipment for liquid chromatography by means of mass spectrometry and ion chromatography) of the State Sub-program of Research In-frastructures and Technical Scientific Equipment. DS Repositorio Institucional de la Universidad de Cádiz RD 05-nov-2026