RT journal article T1 Improving an Industrial Sherry Base Wine by Yeast Enhancement Strategies A1 Ruiz Muñoz, Marina A1 Cordero Bueso, Gustavo Adolfo A1 Izquierdo-Cañas, Pedro Miguel A1 Mena-Morales, Adela A1 Cantoral Fernández, Jesús Manuel A2 BiomedicinaBiotecnología y Salud Pública K1 industrial wine yeasts K1 S. cerevisiae K1 mass-mating K1 adaptive evolution K1 volatile compounds K1 sensory analysis AB There is growing interest in yeast selection for industrial fermentation applications since it is a factor that protects a wine's identity. Although it is strenuous evaluating the oenological characteristics of yeasts in selection processes, in many cases the most riveting yeasts produce some undesirable organoleptic characteristics in wine. The aim of the present work is to improve an industrial yeast strain by reducing its hydrogen sulfide (H2S) production. To accomplish this, two different improvement approaches were used on said yeast: hybridization by mass mating and adaptive laboratory evolution, both performed through spore generation and conjugation, thus increasing genetic variability. Three evolved variants with lower H2S production were obtained and used as starters to carry out fermentation at an industrial level. Wine quality was analyzed by its principal oenological parameters and volatile aroma compounds, which were both corroborated by sensory evaluations. Significant differences between the produced wines have been obtained and a substantial improvement in aromatic quality has been achieved. Both hybrids were the most different to the control due to terpenes and esters production, while the evolved strain was very similar to the parental strain. Not only have organoleptic defects been reduced at an industrial level, more floral and fruitier wines have been produced. PB MDPI SN 2304-8158 YR 2022 FD 2022-04 LK http://hdl.handle.net/10498/26944 UL http://hdl.handle.net/10498/26944 LA eng NO This research was funded by the Centre for the Development of Industrial Technology (CDTI) (grant number IDI-20141202) and the European Regional Development Fund (ERDF) (grant number FEDER-UCA18-106947). DS Repositorio Institucional de la Universidad de Cádiz RD 06-nov-2026