RT journal article
T1 The Need for Standardization in Next-Generation Sequencing Studies for Classic Hodgkin Lymphoma: A Systematic Review
A1 Santiesteban Espejo, Antonio
A1 Bernal-Florindo, Irene
A1 Pérez-Requena, José
A1 Atienza Cuevas, Lidia
A1 Morán Sánchez, Julia
A1 Fernandez-Valle, María del Carmen
A1 Romero‑García, Raquel
A1 García-Rojo, Marcial
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 Medicina
K1 Classic Hodgkin lymphoma
K1 standardization
K1 next-generation sequencing
K1 liquid biopsy
AB Classic Hodgkin lymphoma (cHL) constitutes a B cell-derived neoplasm defined by a scarce tumoral population, termed Hodgkin and Reed-Sternberg (HRS) cells, submerged into a histologically heterogeneous microenvironment. The paucity of HRS cells has historically hampered genetic studies, rendering the identification of the recurrent genetic lesions and molecular pathways deregulated in this lymphoma difficult. The advent of high-throughput sequencing methods such as next-generation sequencing (NGS) could sensibly optimize the identification of the mutational landscape of cHL. However, there is no current consensus either in the design of panels for targeted NGS or in its most relevant clinical applications. In this work, we systematically review the current state of NGS studies of cHL, stressing the need for standardization both in the candidate genes to be analyzed and the bioinformatic pipelines. As different institutions have developed and implemented their own customized NGS-based protocols, to compare and systematically review the major findings of this ongoing research area could be of added value for centers that routinely perform diagnostic, monitoring and genotyping strategies in cHL samples. The results of this systematic review should contribute to the interdepartmental harmonization and achievement of a consensus in the current clinical applications of NGS studies of cHL.
PB MDPI
SN 2075-4418
YR 2022
FD 2022-04
LK http://hdl.handle.net/10498/26958
UL http://hdl.handle.net/10498/26958
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026