RT journal article T1 Influence of Leachate and Nitrifying Bacteria on Photosynthetic Biogas Upgrading in a Two-Stage System A1 Saldarriaga, Luis Fernando A1 Almengló Cordero, Fernando A1 Cantero Moreno, Domingo A1 Ramírez Muñoz, Martín A2 Ingeniería Química y Tecnología de Alimentos K1 photobioreactor K1 biogas upgrading K1 carbon dioxide K1 ammonia removal K1 biomethane K1 gas-liquid ratio K1 leachate K1 microalgae AB Photosynthetic biogas upgrading using two-stage systems allows the absorption of carbon dioxide (CO2) in an absorption unit and its subsequent assimilation by microalgae. The production of microalgae requires large amounts of nutrients, thus making scale-up difficult and reducing economic feasibility. The photosynthetic process produces oxygen (O-2) (1 mol per mol of CO2 consumed), which can be desorbed into purified biogas. Two-stage systems reduce its impact but do not eliminate it. In this study, we test the use of landfill leachate as a nutrient source and propose a viable and economical strategy for reducing the O-2 concentration. First, the liquid/gas (L/G) ratio and flow mode of the absorber were optimized for 20% and 40% CO2 with COMBO medium, then landfill leachate was used as a nutrient source. Finally, the system was inoculated with nitrifying bacteria. Leachate was found to be suitable as a nutrient source and to result in a significant improvement in CO2 absorption, with outlet concentrations of 0.01% and 0.6% for 20% and 40% CO2, respectively, being obtained. The use of nitrifying bacteria allowed a reduction in dissolved oxygen (DO) concentration, although it also resulted in a lower pH, thus making CO2 uptake slightly more difficult. PB MDPI SN 2227-9717 YR 2021 FD 2021-09 LK http://hdl.handle.net/10498/27022 UL http://hdl.handle.net/10498/27022 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 08-nov-2026