RT journal article T1 Start-up of the mesophilic anaerobic co-digestion of two-phase olive-mill waste and cattle manure using volatile fatty acids as process control parameter A1 Rubio, Jose Angel A1 Fernández Güelfo, Luis Alberto A1 Romero García, Luis Isidoro A1 Wilkie, Ann C. A1 García Morales, José Luis A2 Ingeniería Química y Tecnología de Alimentos A2 Tecnologías del Medio Ambiente K1 Anaerobic co-digestion K1 Start-up procedure K1 Two-phase olive-mill waste K1 Cattle manure K1 Propionic acid K1 Mesophilic temperature AB In this work, the start-up and stabilization stages of mesophilic anaerobic co-digestion of 2POMW and CM in asemi-continuously fed stirred tank reactor (SSTR) were analyzed. Volatile fatty acids (VFAs) were monitored andused as the main control parameter for the start-up and stabilization stages, as well as to evaluate the potentialinhibition episodes. The results showed that accumulation of propionic acid was the key factor in the inhibitionof the methanogenic phase, leading to process imbalance. To avoid the problems associated with inhibition byhigh VFA concentrations, several reinoculations were performed using a suitable inoculum adapted to VFAdegradation. The start-up phase was carried out in batch conditions for 97 days, reaching a final concentration ofpropionic acid of 12.77 mg/L. From that moment, the reactor was fed in a semi-continuous mode with a hydraulicretention time (HRT) of 40 days. A total period of 140 days was required to achieve a stable performanceof the reactor with a methane productivity of 0.34 LCH4/LRd. PB ELSEVIER SN 0016-2361 YR 2022 FD 2022-10 LK http://hdl.handle.net/10498/27078 UL http://hdl.handle.net/10498/27078 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 12-nov-2026