RT journal article
T1 Acute Colon Inflammation Triggers Primary Motor Cortex Glial Activation, Neuroinflammation, Neuronal Hyperexcitability, and Motor Coordination Deficits
A1 Carrascal, Livia
A1 Vázquez-Carretero, María D.
A1 García-Miranda, Pablo
A1 Fontán-Lozano, Ángela
A1 Calonge, María L.
A1 Ilundáin, Anunciación A.
A1 Castro González, Carmen
A1 Nunez Abades, Pedro
A1 Peral, María J.
A2 BiomedicinaBiotecnología y Salud Pública
K1 colon inflammation
K1 motor neurons
K1 microglial and astrocyte activation
K1 neuroinflammation
K1 neurodegeneration
K1 hyperexcitability
K1 motor coordination
AB Neuroinflammation underlies neurodegenerative diseases. Herein, we test whether acutecolon inflammation activates microglia and astrocytes, induces neuroinflammation, disturbs neuronintrinsic electrical properties in the primary motor cortex, and alters motor behaviors. We used a ratmodel of acute colon inflammation induced by dextran sulfate sodium. Inflammatory mediators andmicroglial activation were assessed in the primary motor cortex by PCR and immunofluorescenceassays. Electrophysiological properties of the motor cortex neurons were determined by whole-cellpatch-clamp recordings. Motor behaviors were examined using open-field and rotarod tests. Weshow that the primary motor cortex of rats with acute colon inflammation exhibited microglial andastrocyte activation and increased mRNA abundance of interleukin-6, tumor necrosis factor-alpha,and both inducible and neuronal nitric oxide synthases. These changes were accompanied by areduction in resting membrane potential and rheobase and increased input resistance and actionpotential frequency, indicating motor neuron hyperexcitability. In addition, locomotion and motorcoordination were impaired. In conclusion, acute colon inflammation induces motor cortex microglialand astrocyte activation and inflammation, which led to neurons’ hyperexcitability and reduced motorcoordination performance. The described disturbances resembled some of the early features found inamyotrophic lateral sclerosis patients and animal models, suggesting that colon inflammation mightbe a risk factor for developing this disease
PB MDPI
SN 1422-0067
YR 2022
FD 2022-05
LK http://hdl.handle.net/10498/27225
UL http://hdl.handle.net/10498/27225
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026