RT journal article
T1 Extraction of Antioxidant Compounds from Onion Bulb (Allium cepa L.) Using Individual and Simultaneous Microwave-Assisted Extraction Methods
A1 Velasco González de Peredo, Ana
A1 Vázquez Espinosa, Mercedes
A1 Espada Bellido, Estrella
A1 Ferreiro González, Marta
A1 Carrera Fernández, Ceferino Adrián
A1 Fernández Barbero, Gerardo
A1 Palma Lovillo, Miguel
A2 Química Analítica
K1 Allium cepa L.
K1 anthocyanins
K1 Box-Behnken
K1 HPLC
K1 microwave-assisted extraction
K1 onion
K1 phenolic compounds
AB Despite the excellent beneficial properties that anthocyanins and total phenolic compounds give to the red onion bulbs, few articles have investigated modern extraction techniques or experimental designs in this field. For this reason, the present study proposes the development and optimization of alternative methods for the extraction of these compounds based on microwave-assisted extraction and the Box-Behnken experiment design. The optimal values for the extraction of total anthocyanins have been established at 62% methanol composition as a solvent, pH 2, 56 degrees C temperature, and 0.2:13 g:mL sample-solvent ratio. Regarding the extraction of total phenolic compounds, the optimal conditions have been established at 100% pure methanol as a solvent with pH 2, 57 degrees C temperature, and 0.2:8.8 g:mL sample-solvent ratio. Short extraction times (min), good recoveries (mg of bioactive compound g(-1) of dry onion), and high repeatability and intermediate precision (coefficient of variation (%)) have been confirmed for both methods. Regarding total anthocyanins, the following results have been obtained: 2 min, 2.64 +/- 0.093 mg of total anthocyanins g(-1) of dry onion, and 2.51% and 3.12% for precision. Regarding phenolic compounds, the following results have been obtained: 15 min, 7.95 +/- 0.084 mg of total phenolic compound g(-1) of dry onion, and 3.62% and 4.56% for precision. Comparing these results with those of other authors and with those obtained in a previous study of ultrasound-assisted extraction, it can be confirmed that microwave-assisted extraction is a quantitative, repeatable, and very promising method for the extraction of phenolic compounds and anthocyanins, which offers similar and even superior results with little solvent expense, time, and costs
PB MDPI
SN 2076-3921
YR 2022
FD 2022-05
LK http://hdl.handle.net/10498/27240
UL http://hdl.handle.net/10498/27240
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 05-nov-2026