RT journal article T1 Mesophilic anaerobic co-digestion of two-phase olive-mill waste and cattle manure: Optimization of semi-continuous process A1 Rubio Bernal, José Ángel A1 Fernández Güelfo, Luis Alberto A1 Romero García, Luis Isidoro A1 Wilkie, Ann C. A1 García Morales, José Luis A2 Ingeniería Química y Tecnología de Alimentos A2 Tecnologías del Medio Ambiente K1 Anaerobic co-digestion K1 Semi-continuous operation K1 Methane yield K1 Two-phase olive-mill waste K1 Cattle manure AB Anaerobic co-digestion of organic wastes is an effective technology for the management of two or more substrates with different characteristics. In this context, the main objective of this work was the optimization of biogas production for the treatment of a mixture of two-phase olive-mill waste (2POMW) and cattle manure (CM) (60:40 w/w) at mesophilic temperature range (35 degrees C). The effect of hydraulic retention time (HRT) on the performance and stability of the digestion process was studied. A decreasing series of HRTs in the range of 40-12 days was analyzed. The corresponding organic loading rates (OLR) were in the range 2.01-6.07 gVS/L(R)middotd. Pseudo steady-state operation of the reactor was established for HRTs between 40 and 15 days. For 15-days HRT, the maximum values of methane productivity (0.94 LCH4/L(R)middotd) and specific methane yield (0.52 LCH4/gVS(removed)) were obtained while total acidity (measured as acetic acid) in the effluent were < 150 mg/L, verifying process stability. In addition, the removal efficiencies of volatile solids (VS) and dissolved organic carbon (DOC) were 38 and 67 %, respectively. At 12-days HRT, decreases in methane production and organic matter removal efficiencies were observed, with values of 27 and 47 % for VS and DOC removal, respectively. Therefore, 12-days HRT was considered as inadequate for the anaerobic co-digestion of 2POMW and CM because a clear increase in volatile fatty acids was observed at the end of this period, leading to process destabilization and a decrease in biogas production. PB ELSEVIER SN 0016-2361 YR 2022 FD 2022-11 LK http://hdl.handle.net/10498/27448 UL http://hdl.handle.net/10498/27448 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 12-nov-2026