RT journal article
T1 Evaluation of the Effect of Different Co-Solvent Mixtures on the Supercritical CO2 Extraction of the Phenolic Compounds Present in Moringa oleifera Lam. Leaves
A1 Yerena-Prieto, Beatriz Juliana
A1 Gonzalez-Gonzalez, Montserrat
A1 Garcia Alvarado, Miguel Angel
A1 Casas Cardoso, Lourdes
A1 Palma Lovillo, Miguel
A1 Rodríguez-Jimenes, Guadalupe del Carmen
A1 Fernández Barbero, Gerardo
A1 Cejudo Bastante, Cristina
A2 Ingeniería Química y Tecnología de Alimentos
A2 Química Analítica
K1 Moringa olifera Lam
K1 green technology
K1 supercritical fluid extraction
K1 co-solvent
K1 phenolic compounds
K1 flavonoids
AB Supercritical fluid extraction (SFE), using CO2, is a novel, sustainable and very efficient technique for the recovery of highly apolar compounds. However, the recovery of phenolic compounds requires the use of different co-solvent combinations such as water and ethanol to enhance the recovery of these compounds through the optimization of a number of variables. In this sense, the effect of pressure (100, 150 and 200 bar), temperature (50, 65 and 80 degrees C), extraction time (30, 60, 90, 120, 150 and 180 min) and the effect of the different percentages of ethanol and water as co-solvents on the composition and phenolic content of moringa leaf extracts were evaluated. Six major flavonoids were identified by ultra-high-performance liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometer (UHPLC-Q-ToF-MS). Pressure and temperature had a significant effect on the phenolic composition of the extracts, as well as on their concentrations. The highest concentration of total flavonoids compounds (TFCs) was obtained by using a mixture of CO2 and water of 50:50 (v/v) at 100 bar, at 65 degrees C after a 120 min extraction time that produced a concentration of 11.66 mg +/- 0.02 mg TFC g(-1) sample, which corresponds to 89.0% of the total flavonoids of the sample, obtained by exhaustive extraction.
PB ELSEVIER
SN 2073-4395
YR 2022
FD 2022-06
LK http://hdl.handle.net/10498/27508
UL http://hdl.handle.net/10498/27508
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026