RT journal article
T1 Supercritical Impregnation of Mango Leaf Extract into PLA 3D-Printed Devices and Evaluation of Their Biocompatibility with Endothelial Cell Cultures
A1 Grosso, Pilar
A1 Cejudo Bastante, Cristina
A1 Sánchez Gomar, Ismael
A1 Durán Ruiz, María del Carmen
A1 Moreno Luna, Rafael
A1 Casas Cardoso, Lourdes
A1 Pereyra López, Clara María
A1 Mantell Serrano, Casimiro
A2 BiomedicinaBiotecnología y Salud Pública
A2 Ingeniería Química y Tecnología de Alimentos
K1 supercritical impregnation
K1 mango leaf
K1 3D printer
K1 PLA
K1 ECFCs
AB The addition of natural substances with pharmacoactive properties to polymeric biomedical devices would provide beneficial regarding the assimilation of these endoprostheses when implanted into a patient's body. The added drug would facilitate endothelization by regulating the inflammatory processes that such interventions entail, preventing contamination hazards and favoring the angiogenesis or formation of blood vessels in the tissue. The present work used mango leaf extract (MLE) obtained through pressurized ethanol for this purpose. Polylactic acid (PLA) in the form of filaments or 3D-printed disks was impregnated by means of supercritical technology with MLE for the culture essays. The release kinetics has been studied and the polymer matrices have been examined by scanning electron microscopy (SEM). The impregnated devices were subjected to in vitro culture of colony-forming endothelial cells. The influence of the different impregnation conditions used for the production of the MLE impregnated polymeric devices on the development of the cell culture was determined by fluorescence microscopy. The best results were obtained from the calcein cultures on 35 degrees C MLE impregnated into 3D-printed polymer disks.
PB MDPI
SN 2073-4360
YR 2022
FD 2022-07
LK http://hdl.handle.net/10498/27553
UL http://hdl.handle.net/10498/27553
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026