RT journal article
T1 Automatable downstream purification of the benzohydroxamic acid D-DIBOA from a biocatalytic synthesis
A1 Calle Sierra, María Elena de la
A1 Cabrera Revuelta, Gema
A1 Linares-Pineda, Teresa
A1 Cantero Moreno, Domingo
A1 González Molinillo, José María
A1 Varela Montoya, Rosa María
A1 Valle Gallardo, Antonio
A1 Bolívar Pérez, Jorge
A2 BiomedicinaBiotecnología y Salud Pública
A2 Ingeniería Química y Tecnología de Alimentos
A2 Química Orgánica
K1 D-DIBOA
K1 Green chemistry
K1 Whole-cell biocatalyst
K1 Downstream processes
K1 Chromatography
K1 Ecological herbicides
AB Herbicides play a vital role in agriculture, contributing to increased crop productivity by minimizing weed growth, but their low degradability presents a threat to the environment and human health. Allelochemicals, such as DIBOA (2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4 H)-one), are secondary metabolites released by certain plants that affect the survival or growth of other organisms. Although these metabolites have an attractive po-tential for use as herbicides, their low natural production is a critical hurdle. Previously, the synthesis of the biologically active analog D-DIBOA (4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one) was achieved, using an engi-neered E. coli strain as a whole-cell biocatalyst, capable of transforming a precursor compound into D-DIBOA and exporting it into the culture medium, although it cannot be directly applied to crops. Here a chromatographic method to purify D-DIBOA from this cell culture medium without producing organic solvent wastes is described. The purification of D-DIBOA from a filtered culture medium to the pure compound could also be automated. Biological tests with the purified compound on weed models showed that it has virtually the same activity than the chemically synthesized D-DIBOA.
PB ELSEVIER
SN 1876-4347
YR 2022
FD 2022-12
LK http://hdl.handle.net/10498/27572
UL http://hdl.handle.net/10498/27572
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026