RT journal article
T1 Survival of Atlantic bluefin tuna (Thunnus thynnus) larvae hatched at different salinity and pH conditions
A1 Ruiz Jarabo de la Rocha, Ignacio
A1 Laiz Carrión, Raúl
A1 Ortega, A.
A1 Gándara, F.
AD  de la
A1 Quintanilla Hervás, José María
A1 Mancera Romero, Juan Miguel
A2 Biología
K1 Atlantic bluefin tuna
K1 Larvae
K1 pH
K1 Salinity
K1 Thunnus thynnus
AB In this study, we assessed the effect of environmental salinity and pH as independent factors on larval survival of Atlantic bluefin tuna (ABFT –Thunnus thynnus) together with their whole-body Na+/K+-ATPase and v-type H+-ATPase activities. Fertilized eggs of ABFT were obtained from a spontaneous spawning of broodstock in the farming facilities at El Gorguel (Cartagena, SE Spain) and were transferred to facilities of the Spanish Institute of Oceanography (IEO) in Mazarrón (SE Spain). In a first experiment, eggs (200 fertilized eggs L−1 per treatment, in 3 replicates) were exposed to different salinities treatments and constant pH 8.0 (control) until hatch was completed (50 h post-fertilization, hpf, at 23 °C): 27, 30, 33, 36, 37, 38 (control), 39, 40, 43, 46 and 49 ppt. In a second experiment eggs (200 fertilized eggs L−1, in 3 replicates) were exposed to seawater salinity (SW: 38 ppt) and four reduced pH treatments until hatch was completed (50 hpf at 23 °C): 8.0 (control), 7.7, 7.5 and 7.3. An inverse “U-shaped” relationship was observed between environmental salinity and number of hatched larvae. An opposite pattern was observed for both Na+/K+-ATPase and H+-ATPase activities in hatched larvae, increasing both activities in groups exposed to extreme salinities. Thus, larval survival was higher at intermediate salinities and lower at the extreme salinities tested. These results suggest higher survival rates with lower active pumps activities. No significant differences in larval survival were observed with pH treatment, but lower H+-ATPase activity was detected at control environmental pH (pH 8.0). Survival results are discussed in terms of osmoregulatory cost adapting to a salinity and pH predicted for the near future scenarios. © 2022
PB ELSEVIER
SN 0044-8486
YR 2022
FD 2022-11
LK http://hdl.handle.net/10498/27596
UL http://hdl.handle.net/10498/27596
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026