RT journal article
T1 Spexin in the European sea bass, Dicentrarchus labrax: Characterization, brain distribution, and interaction with Gnrh and Gnih neurons
A1 Paullada Salmerón, José Antonio
A1 Wang, Bin
A1 Muñoz Cueto, José Antonio
A2 Biología
K1 Gnih
K1 Gnrh
K1 immunohistochemistrym
K1 reproduction
K1 sea bass
K1 spexin
AB Spexin (Spx) is a recently characterized neuropeptide implicated in multiple physiologicalprocesses in vertebrates, including reproduction, food intake, and regulationof anxiety and stress. Two orthologs (Spx1 and Spx2) are present in some nonmammalianvertebrates, including teleosts. However, information on the distribution ofSpx in the brain and its interactions with other neuroendocrine systems in fish isstill scarce. In this work, we cloned and sequenced the sea bass (Dicentrarchus labrax)Spx1, which included a 27 aa signal peptide and a mature peptide of 14 aa that isC-terminal amidated. spx1 transcripts were higher in the diencephalon/caudal preopticarea/hypothalamus and medulla but were also detected in the olfactory bulbs,telencephalon/rostral preoptic area, optic tectum/tegmentum, cerebellum/pons, andpituitary. The immunohistochemical study revealed Spx1-immunoreactive (ir) cells indifferent nuclei of the preoptic area, habenula, prethalamus, mesencephalic tegmentumand in the proximal pars distalis (PPD) and pars intermedia of the pituitary. Spx1-irfibers were widely distributed throughout the brain being particularly abundant inthe midbrain and hindbrain, in close contact with tegmental gonadotropin-releasinghormone 2 (Gnrh2) cells and isthmic gonadotropin-inhibitory hormone (Gnih) cells ofthe secondary gustatory nucleus. Moreover, Gnih fibers were observed innervatingSpx1-ir cells lying in several subdivisions of the magnocellular preoptic nucleus andin the lateral nucleus of the valvula, whereas ventrolateral prethalamic Spx1-ir cellsreceived immunopositive Gnrh2 fibers. In the pituitary, Gnrh1-ir fibers were observedclosely associated with Spx1-ir cells of the PPD. These results suggest that Spx1 couldbe involved in both reproductive and nonreproductive (i.e., food intake, behavior)functions in sea bass.
PB WILEY
SN 1096-9861
YR 2022
FD 2022-10
LK http://hdl.handle.net/10498/27710
UL http://hdl.handle.net/10498/27710
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026