RT journal article
T1 A New Electrochemical Method to Determine Tryptophan in Fruit Juices: Development and Validation
A1 Takele, Assefa
A1 Palacios Santander, José María
A1 Palma Lovillo, Miguel
A2 Química Analítica
K1 tryptophan
K1 ultra performance liquid chromatography
K1 differential pulse voltammetry
K1 sonogel–carbon electrodes
K1 fruit juice
AB Tryptophan (Trp) is an essential amino acid usually found in fruit juices. Its determination is necessary for food companies because of its relation to human health. In this work, a new electrochemical method based on sonogel-carbon electrodes (SNGCEs) was developed and validated using an ultra performance liquid chromatography (UPLC) method as a reference method for the determination of Trp in fruit juices. Cyclic voltammetry (CV), chronoamperometry, and differential pulse voltammetry (DPV) techniques were applied to investigate the oxidation of Trp on a previously polarized SNGCE surface in a Britton-Robinson (BR) buffer solution at pH 3.6. The operating conditions for electroanalysis were optimized using a Box-Behnken design (BBD), obtaining an oxidation peak for Trp at 0.749 V. The linear range for this method was from 0.1 to 5 mg/L. The intraday and interday precision, expressed as a relative standard deviation (RSD), were 3.1% and 2.7%, respectively. The average recovery was 99.01%, and the limit of detection and quantitation were 0.33 and 1.09 mg/L, respectively. Therefore, from the quality analytical parameters obtained, it can be concluded that the new electrochemical method can be successfully used for the routine analysis of Trp in fruit juices. As far as we are concerned, this is the first time that a methodology for Trp determination was performed in this kind of real food matrices.
PB MDPI
SN 2304-8158
YR 2022
FD 2022-07
LK http://hdl.handle.net/10498/27873
UL http://hdl.handle.net/10498/27873
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026