RT journal article
T1 How Meaningful Are Minor Details in the Generation of Nanomodified Electrochemical Enzyme Biosensors? Exploring the Scenario with Sinusoidal Approaches
A1 Rahman, Md. Towhidur
A1 López-Iglesias, David
A1 Sierra Padilla, Alfonso
A1 García Guzmán, Juan José
A1 Cubillana Aguilera, Laura
A1 Bellido Milla, Dolores
A1 Palacios Santander, José María
A2 Química Analítica
K1 Sonogel-Carbon (bio)sensors
K1 gold nanoparticles
K1 carbon nanotubes
K1 tyrosinase
K1 sinusoidal methods
K1 3,4-ethylenedioxythiophene
K1 dopamine
AB In this work, a screening of Sonogel-Carbon (SNGC) electrodes modified with nanomaterials (carbon nanotubes and gold nanoparticles) and the study of their effect on the electrochemical performance of sinusoidal voltage (SV) and current (SC)-based biosensors are reported. Surface modification was achieved by drop-casting and electrodeposition methodologies. Within the strategies used, SV and SC, recently exploited procedures, were used to electrodeposit simultaneously a poly 3,4-ethylenedioxythiophene (PEDOT)-tyrosinase layer and the corresponding nanostructured material. Dopamine was selected as a benchmark analyte to evaluate the analytical performance of the different (bio)sensors obtained in terms of relevant figures of merit, such as sensitivity, limits of detection and quantitation, and accuracy, among others. A discussion about the pros and cons between the type of modification and the methods employed is also presented. Briefly, SC based sensors offered excellent quality analytical parameters and lower dispersion of the results. They were employed for more specific electrochemical studies, including interferences assays and the determination of DA in real samples, obtaining good recoveries (101-110.6%). The biosensor modified with gold nanoparticles (AuNPs) (drop-casting method) and SC-electrodeposited showed the best figures of merit: R-2 = 0.999; sensitivity = -4.92 x 10(-9) A.mu M-1; RSDsensitivity = 1.60%; LOD = 5.56 mu M; RSDLOD = 6.10%; and LOQ = 18.53 mu M.
PB MDPI
SN 2227-9040
YR 2022
FD 2022-08
LK http://hdl.handle.net/10498/28059
UL http://hdl.handle.net/10498/28059
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026