RT journal article
T1 Potential use of Bacillus paramycoides for the production of the biopolymer polyhydroxybutyrate from leftover carob fruit agro-waste
A1 Djerrab, Leila
A1 Chekroud, Zohra
A1 Rouabhia, Amer
A1 Dems, Mohamed Abdesselem
A1 Attailia, Imane
A1 Romero García, Luis Isidoro
A1 Smadi, Mustapha Adnane
A2 Ingeniería Química y Tecnología de Alimentos
K1 Bacillus paramycoides
K1 carob waste
K1 crotonic acid
K1 polyhydroxybutyrate (PHB)
K1 valorization
AB This study was designed to investigate, at a laboratory scale, the possibility of valorizing the leftover carob fruits to produce the eco-friendly biopolymer polyhydroxybutyrate (PHB) by using the bacterial strain Bacillus paramycoides, which has been isolated from the botanical garden of Skikda University in Algeria. The PHB production was tested under various conditions: a pH of 3-8, temperature range of 30-44 celcius, carob extracted molasses concentration of 2-8% v/v, an incubation time of 24-96 h and an agitation speed of 150-300 rpm. The effects of different nitrogen sources and carob extracted molasses treatment types were also investigated. The PHB concentration was determined quantitatively as crotonic acid by measuring the absorbance at 300 nm. Cell growth was quantified by measuring the density of the culture at 600 nm. The presence of PHB was confirmed by applying high-performance liquid chromatography (HPLC) using an Aminex HPX-87H and implementing gas chromatography analysis. The best yield of PHB synthesis was obtained by using 6% v/v of 5 M H2SO4 treated with carob molasses as a carbon source, with peptone as a nitrogen source; incubation was conducted at 37 degrees C for 96 hat an agitation speed of 300 rpm (114.95 mg/L). The HPLC analysis confirmed the synthesis of PHB by B. paramycoides to have a chromatogram retention time of 22.5 min. Carob waste was successfully valorized to PHB.
PB AMER INST MATHEMATICAL SCIENCES-AIMS
SN 2471-1888
YR 2022
FD 2022
LK http://hdl.handle.net/10498/28247
UL http://hdl.handle.net/10498/28247
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026