RT journal article
T1 Signaling pathways activated by sea bass gonadotropin-inhibitory hormone peptides in COS-7 cells transfected with their cognate receptor
A1 Wang, Bin
A1 Paullada Salmerón, José Antonio
A1 Verges Castillo, Alba
A1 Gómez, Ana
A1 Muñoz Cueto, José Antonio
A2 Biología
K1 GnIH
K1 GnIH receptor
K1 kisspeptin
K1 kisspeptin receptor
K1 signaling pathway
AB Results of previous studies provided evidence for the existence of a functional gonadotropin-inhibitory hormone (GnIH) system in the European sea bass, Dicentrarchus labrax, which exerted an inhibitory action on the brain-pituitary-gonadal axis of this species. Herein, we further elucidated the intracellular signaling pathways mediating in sea bass GnIH actions and the potential interactions with sea bass kisspeptin (Kiss) signaling. Although GnIH1 and GnIH2 had no effect on basal CRE-luc activity, they significantly decreased forskolin-elicited CRE-luc activity in COS-7 cells transfected with their cognate receptor GnIHR. Moreover, an evident increase in SRE-luc activity was noticed when COS-7 cells expressing GnIHR were challenged with both GnIH peptides, and this stimulatory action was significantly reduced by two inhibitors of the PKC pathway. Notably, GnIH2 antagonized Kiss2-evoked CRE-luc activity in COS-7 cells expressing GnIHR and Kiss2 receptor (Kiss2R). However, GnIH peptides did not alter NFAT-RE-luc activity and ERK phosphorylation levels. These data indicate that sea bass GnIHR signals can be transduced through the PKA and PKC pathways, and GnIH can interfere with kisspeptin actions by reducing its signaling. Our results provide additional evidence for the understanding of signaling pathways activated by GnIH peptides in teleosts, and represent a starting point for the study of interactions with multiple neuroendocrine factors on cell signaling.
PB FRONTIERS MEDIA SA
SN 1664-2392
YR 2022
FD 2022-08
LK http://hdl.handle.net/10498/28264
UL http://hdl.handle.net/10498/28264
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026