RT journal article T1 Non-Saccharomyces Are Also Forming the Veil of Flor in Sherry Wines A1 Ruiz Muñoz, Marina A1 Hernández Fernández, María A1 Cordero Bueso, Gustavo Adolfo A1 Martínez-Verdugo, Sergio A1 Pérez, Fernando A1 Cantoral Fernández, Jesús Manuel A2 BiomedicinaBiotecnología y Salud Pública K1 non-Saccharomyces K1 Pichia spp. K1 Sherry wines K1 biofilm K1 metabolic properties K1 extracellular enzymes K1 biological ageing AB Biological ageing is an essential process for obtaining some distinctive Sherry wines, such as Fino and Manzanilla. It occurs after the fermentation of the grape must due to the appearance of a biofilm on the surface of the wine called "veil of flor". Yeasts belonging to the Saccharomyces cerevisiae species mainly comprise such biofilm. Although other species have also been found, these have been traditionally considered spoilage. Indeed, it has even been hypothesised that they may not be able to form biofilm on their own under such conditions. In the present work, four different non-Saccharomyces yeasts isolated from barrels in the Jerez area under biological ageing have been characterised through their physiological abilities, including extracellular enzymatic and biofilm-forming capabilities. Results showed not only a surprising ethanol tolerance, above 15.5% in all cases, but also a significant degree of extracellular enzyme production, highlighting the urease and proteolytic activities found in Pichia manshurica, as well as lipolytic activity in Pichia kudriavzevii, Pichia membranifaciens and Wicherhamomyces anomalus. As a conclusion, these non-Saccharomyces could be very interesting in the oenological field, beyond improving the organoleptic characteristics as well as technological features in these wines. PB MDPI SN 2311-5637 YR 2022 FD 2022-09 LK http://hdl.handle.net/10498/28364 UL http://hdl.handle.net/10498/28364 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 06-nov-2026