RT journal article
T1 Application of a Microalgal Peptide-Enriched Extract as Media Component in E. coli Culture
A1 Callejo-Lopez, J.A.
A1 Ramírez Muñoz, Martín
A1 González Cortés, José Joaquín
A1 Valle Gallardo, Antonio
A1 Cantero Moreno, Domingo
A1 Bolívar Pérez, Jorge
A2 BiomedicinaBiotecnología y Salud Pública
A2 Ingeniería Química y Tecnología de Alimentos
K1 microalgae
K1 peptide-enriched extract
K1 culture medium
K1 E. coli
K1 succinate
AB This study evaluated the feasibility of applying a microalgal peptide-enriched extract, obtained through a sequential alkaline and enzymatic method, as a fundamental media component in Escherichia coli culture. The results demonstrated that the extract is viable as the primary nutrient source for E. coli cultures (wild type), even as a complete substitute for the widely used Luria-Bertani (LB) medium, using an extract dilution of 1:3. Its application in a proportion carbon limiting (dilution 1:7), with glycerol supplementation at the early stationary phase, improved the nutrient use efficiency and controlled the undesirable acetate production. Furthermore, this last strategy applied in an E. coli mutant (M4-Delta iclR) enhanced the production of succinate by 67.3% compared with the M9 medium. The innovative approach proposed in this study opens a wide range of applications and studies related to the production of bio-compounds through diverse microorganisms.
PB AMER CHEMICAL SOC
SN 3529-3538
YR 2023
FD 2023-02
LK http://hdl.handle.net/10498/28399
UL http://hdl.handle.net/10498/28399
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026