RT journal article
T1 Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
A1 Cardoza, Rosa Elena
A1 McCormick, Susan P.
A1 Izquierdo-Bueno Reina, Inmaculada Concepción
A1 Martínez-Reyes, Natalia
A1 Lindo, Laura
A1 Brown, Daren W
A1 González Collado, Isidro
A1 Proctor, Robert H.
A1 Gutiérrez, Santiago
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Orgánica
K1 Aspinolides
K1 Trichoderma
K1 Trichothecenes
K1 Metabolomics
K1 Gene clusters
K1 Comparative genomics
AB The fungus Trichoderma arundinaceum exhibits biological control activity against crop diseases caused by other fungi. Two mechanisms that likely contribute to this activity are upregulation of plant defenses and production of two types of antifungal secondary metabolites: the sesquiterpenoid harzianum A (HA) and the polyketide-derived aspinolides. The goal of the current study was to identify aspinolide biosynthetic genes as part of an effort to understand how these metabolites contribute to the biological control activity of T. arundinaceum. Comparative genomics identified two polyketide synthase genes (asp1 and asp2) that occur in T. arundinaceum and Aspergillus ochraceus, which also produces aspinolides. Gene deletion and biochemical analyses in T. arundinaceum indicated that both genes are required for aspinolide production: asp2 for formation of a 10-member lactone ring and asp1 for formation of a butenoyl subsituent at position 8 of the lactone ring. Gene expression and comparative genomics analyses indicated that asp1 and asp2 are located within a gene cluster that occurs in both T. arundinaceum and A. ochraceus. A survey of genome sequences representing 35 phylogenetically diverse Trichoderma species revealed that intact homologs of the cluster occurred in only two other species, which also produced aspinolides. An asp2 mutant inhibited fungal growth more than the wild type, but an asp1 mutant did not, and the greater inhibition by the asp2 mutant coincided with increased HA production. These findings indicate that asp1 and asp2 are aspinolide biosynthetic genes and that loss of either aspinolide or HA production in T. arundinaceum can be accompanied by increased production of the other metabolite(s).
PB SPRINGER
SN 1432-0614
YR 2022
FD 2022-11
LK http://hdl.handle.net/10498/28444
UL http://hdl.handle.net/10498/28444
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026