RT journal article
T1 Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
A1 Reyes Peces, María Virtudes
A1 Félix Ruiz, Eduardo José
A1 Martínez Vázquez, Francisco
A1 Fernández Montesinos, Rafael
A1 Bomati Miguel, Óscar
A1 Mesa Díaz, María del Mar
A1 Alcántara Puerto, Rodrigo
A1 Vilches Pérez, José Ignacio
A1 Salido Peracaula, Mercedes
A1 Rosa Fox, Nicolás Daniel de la
A1 Piñero de los Ríos, Manuel
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 Física de la Materia Condensada
A2 Ingeniería Química y Tecnología de Alimentos
A2 Materno-Infantil y Radiología
A2 Química Física
K1 3D scaffold
K1 hybrid
K1 sol-gel ink
K1 robocasting
K1 laser micromachining
K1 osteoblasts
K1 regenerative medicine
K1 bone tissue engineering
K1 cytoskeleton
K1 focal adhesion
AB The design and synthesis of sol-gel silica-based hybrid materials and composites offer significant benefits to obtain innovative biomaterials with controlled porosity at the nanostructure level for applications in bone tissue engineering. In this work, the combination of robocasting with sol-gel ink of suitable viscosity prepared by mixing tetraethoxysilane (TEOS), gelatin and beta-tricalcium phosphate (beta-TCP) allowed for the manufacture of 3D scaffolds consisting of a 3D square mesh of interpenetrating rods, with macropore size of 354.0 +/- 17.0 mu m, without the use of chemical additives at room temperature. The silica/gelatin/beta-TCP system underwent irreversible gelation, and the resulting gels were also used to fabricate different 3D structures by means of an alternative scaffolding method, involving high-resolution laser micromachining by laser ablation. By this way, 3D scaffolds made of 2 mm thick rectangular prisms presenting a parallel macropore system drilled through the whole thickness and consisting of laser micromachined holes of 350.8 +/- 16.6-micrometer diameter, whose centers were spaced 1312.0 +/- 23.0 mu m, were created. Both sol-gel based 3D scaffold configurations combined compressive strength in the range of 2-3 MPa and the biocompatibility of the hybrid material. In addition, the observed Si, Ca and P biodegradation provided a suitable microenvironment with significant focal adhesion development, maturation and also enhanced in vitro cell growth. In conclusion, this work successfully confirmed the feasibility of both strategies for the fabrication of new sol-gel-based hybrid scaffolds with osteoconductive properties.
PB MDPI
SN 2310-2861
YR 2022
FD 2022-10
LK http://hdl.handle.net/10498/28688
UL http://hdl.handle.net/10498/28688
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026