RT journal article
T1 Evaluation of Different Extraction Methods on the Phenolic Profile and the Antioxidant Potential of Ceratonia siliqua L. Pods Extracts
A1 El Mansouri, Fouad
A1 Esteves Silva, Joaquim C. G.
A1 Cacciola, Francesco
A1 Asraoui, Fadoua
A1 Tayeq, Hatim
A1 Mttougui Ben Amar, Yasmine
A1 Palma Lovillo, Miguel
A1 Chouaibi, Noureddine
A1 Brigui, Jamal
A2 Química Analítica
K1 Ceratonia siliqua L.
K1 pods
K1 polyphenols
K1 flavonoids
K1 tannins
K1 antioxidant activity
K1 extraction methods
K1 HPLC
AB The present work was designed to investigate the effects of different extraction processes, namely ultrasonic-assisted, supercritical fluid, microwave-assisted and Soxhlet applied to carob pods. The total phenolic quantification and the antioxidant activity were assessed by the means of rapid in vitro spectrophotometric assays; the phenolic profile was identified using ultra-high performance liquid chromatography coupled to mass spectrometry. The results revealed that the phenolic compounds and the antioxidant capacity varied significantly with the nature of the extraction process. The content of total phenolic compounds ranged from 11.55 to 34.38 mg GAE/g DW; the content of total flavonoids varied from 3.50 to 10.53 mg QE/g DW, and the content of condensed tannins fluctuated from 3.30 to 6.55 mg CE/ g DW. All extracts performed differently on antioxidant activity when determined by the DPPH assay producing a dose-dependent response, with IC50 extended from 11.33 to 6.07 mu g/mL. HPLC analysis enabled the identification of nine compounds. As a function of the studied extraction methods, the phenolic compound contents were positively correlated with antioxidant activity.
PB MDPI
SN 1420-3049
YR 2022
FD 2022-10
LK http://hdl.handle.net/10498/28821
UL http://hdl.handle.net/10498/28821
LA eng
NO The Portuguese "Fundacao para a Ciencia e Tecnologia" (FCT, Lisbon) is acknowledged for funding the R&D Units CIQUP (UIDB/000081/2020) and the Associated Laboratory IMS (LA/P/0056/2020).
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026