RT journal article
T1 The antibacterials ciprofloxacin, trimethoprim and sulfadiazine modulate gene expression, biomarkers and metabolites associated with stress and growth in gilthead sea bream (Sparus aurata).
A1 Fernandez, Ronield
A1 Colás Ruiz, Nieves del Rocio
A1 Martínez Rodríguez, Gonzalo
A1 Lara Martín, Pablo Antonio
A1 Mancera Romero, Juan Miguel
A1 Trombini, Chiara
A1 Blasco, Julián
A1 Hampel, Miriam
A2 Biología
A2 Química Física
K1 Antibacterial
K1 Gene expression
K1 Hypothalamic-pituitary axes
K1 Oxidative stress
K1 Sparus aurata
AB The high consumption and subsequent input of antibacterial compounds in marine ecosystems has become a worldwide problem. Their continuous presence in these ecosystems allows a direct interaction with aquatic organisms and can cause negative effects over time. The objective of the present study was to evaluate the effects of exposure to three antibacterial compounds of high consumption and presence in marine ecosystems (Ciprofloxacin CIP, Sulfadiazine SULF and Trimethoprim TRIM) on the physiology of the gilthead sea bream, Sparus aurata. Plasma parameters, enzymatic biomarkers of oxidative stress and damage and expression of genes related to stress and growth were assessed in exposed S. aurata specimens. For this purpose, sea bream specimens were exposed to individual compounds at concentrations of 5.2 ± 2.1 μg L−1 for CIP, 3.8 ± 2.7 μg L−1 for SULF and 25.7 ± 10.8 μg L−1 for TRIM during 21 days. Exposure to CIP up-regulated transcription of genes associated with the hypothalamic-pituitary-thyroid (HPT) (thyrotropin-releasing hormone, trh) and hypothalamic-pituitary-interrenal (HPI) axes (corticotropin-releasing hormone-binding protein, crhbp) in the brain, as well as altering several hepatic stress biomarkers (catalase, CAT; glutathione reductase, GR; and lipid peroxidation, LPO). Similar alterations at the hepatic level were observed after exposure to TRIM. Overall, our study indicates that S. aurata is vulnerable to environmentally relevant concentrations of CIP and TRIM and that their exposure could lead to a stress situation, altering the activity of antioxidant defense mechanisms as well as the activity of HPT and HPI axes
PB Elsevier
SN 0166-445X
YR 2022
FD 2022-07-16
LK http://hdl.handle.net/10498/29413
UL http://hdl.handle.net/10498/29413
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026