RT journal article
T1 Potential application for antimicrobial and antileukemic therapy of a flavonoid-rich fraction of Camellia sinensis
A1 Corrêa de Souza, Mariana
A1 de Souza Mesquita, Leonardo M.
A1 Pena, Fabíola L.
A1 Tamborlin, Leticia
A1 Capelasso da Silva, Laise
A1 Viganó, Juliane
A1 Costa Antunes, Adriane E
A1 Luchessi, Augusto D.
A1 Teixeira Duarte, Marta C.
A1 Fernández Barbero, Gerardo
A1 Ariel Rostagno, Mauricio
A2 Química Analítica
K1 Coupled extraction techniques
K1 Flavonoids
K1 Theaflavin
K1 Cancer therapy
K1 THP-1
AB The antimicrobial and antileukemic effect of a purified fraction of flavonoids from the leaves of Camellia sinensis was evaluated. An extraction yield of 9.77 mg.g-1 total flavonoids was recovered through a pressurized liquid extraction associated with solid-phase extraction. This fraction was tested against pathogenic microorganisms (Staphylococcus, Salmonella, and Enterococcus), considering the minimum inhibitory concentration. In addition, the human monocyte cell line THP-1, derived from a patient with acute monocytic leukemia, was used for the antitumor assay. The results show that the flavonoid-rich fraction obtained by coupling a Pressurized Liquids Extraction in-line with a Solid Phase Extraction (PLE-SPE) has a high antimicrobial effect and resulted in cell cycle blockage G0 / G1, increased DNA fragmentation, and altered leukemic cell morphology. These results suggest that a flavonoid-rich fraction obtained from Camellia sinensis can be applied as potential adjuvants in chemotherapy treatment to mitigate the side effects caused by chemotherapy or even as a supplement to cancer therapy
PB Elsevier
SN 2772-753X
YR 2022
FD 2022-05-18
LK http://hdl.handle.net/10498/29445
UL http://hdl.handle.net/10498/29445
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026