RT journal article
T1 Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
A1 Gómez‑González, Emilio
A1 Muñoz, Olga
A1 Gómez Martín, Juan Carlos
A1 Aceituno‑Castro, Jesús
A1 Fernández‑Muñoz, Beatriz
A1 Navas‑García, José Manuel
A1 Barriga‑Rivera, Alejandro
A1 Fernández‑Lizaranzu, Isabel
A1 Munoz‑González, Francisco Javier
A1 Parrilla‑Giráldez, Rubén
A1 Requena‑Lancharro, Desiree
A1 Gil‑Gamboa, Pedro
A1 Ramos, José Luis
A1 Rosell-Valle, Cristina
A1 Gómez‑González, Carmen
A1 Martín‑López, María
A1 Relimpio López, María Isabel
A1 Perales‑Esteve, Manuel A.
A1 Puppo‑Moreno, Antonio
A1 García Cózar, Francisco José
A1 Olvera Collantes, Lucía
A1 de los Santos‑Trigo, Silvia
A1 Gómez, Emilia
A1 Sánchez Pernaute, Rosario
A1 Padillo‑Ruiz, Javier
A1 Márquez‑Rivas, Javier
A2 BiomedicinaBiotecnología y Salud Pública
K1 COVID-19
K1 Mueller polarimetry
K1 Polarimetric imaging
K1 SARS-CoV-2
K1 Virus detection
AB Objective: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. Approach: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. Main results: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. Significance: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities.
PB Elsevier
SN 0022-4073
YR 2023
FD 2023-03-06
LK http://hdl.handle.net/10498/29550
UL http://hdl.handle.net/10498/29550
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026