RT journal article
T1 A chromosome-level genome assembly enables the identification of the follicule stimulating hormone receptor as the master sex-determining gene in the flatfish Solea senegalensi
A1 de la Herrán, Roberto
A1 Hermida, Miguel
A1 Rubiolo, Juan Andres
A1 Gómez Garrido, Jèssica
A1 Cruz, Fernando
A1 Robles, Francisca
A1 Navajas Pérez, Rafael
A1 Blanco, Andres
A1 Rodriguez Villamayor, Paula
A1 Torres, Dorinda
A1 Sánchez Quinteiro, Pablo
A1 Ramírez Torres, Daniel
A1 Rodríguez Jiménez, María Esther
A1 Arias Pérez, Alberto
A1 Cross Pacheco, Ismael
A1 Duncan, Neil
A1 Martínez Peña, Teresa
A1 Riaza, Ana
A1 Millán, Adrian
A1 De Rosa, M. Cristina
A1 Pirolli, Davide
A1 Gut, Marta
A1 Bouza, Carmen
A1 Robledo, Diego
A1 Rebordinos González, Laureana
A1 Alioto, Tyler
A1 Ruíz  Rejón, Carmelo
A1 Martínez, Paulino
A2 BiomedicinaBiotecnología y Salud Pública
K1 follicule stimulating hormone receptor
K1 genetic map
K1 Senegalese sole
K1 sex determination
K1 whole genome sequencing
AB Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized by its adaptation to demersal life, compact genomes and diversity of SD mechanisms. Here, we assembled the Solea senegalensis genome, a flatfish of great commercial value, into 82 contigs (614 Mb) combining long- and short-read sequencing, which were next scaffolded using a highly dense genetic map (28,838 markers, 21 linkage groups), representing 98.9% of the assembly. Further, we established the correspondence between the assembly and the 21 chromosomes by using BAC-FISH. Whole genome resequencing of six males and six females enabled the identification of 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) consistent with an XX/XY SD system. The observed sex association was validated in a broader independent sample, providing a novel molecular sexing tool. The fshr gene displayed differential expression between male and female gonads from 86 days post-fertilization, when the gonad is still an undifferentiated primordium, concomitant with the activation of amh and cyp19a1a, testis and ovary marker genes, respectively, in males and females. The Y-linked fshr allele, which included 24 nonsynonymous variants and showed a highly divergent 3D protein structure, was overexpressed in males compared to the X-linked allele at all stages of gonadal differentiation. We hypothesize a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
PB Wiley
SN 1755-098X
YR 2023
FD 2023-01-01
LK http://hdl.handle.net/10498/29686
UL http://hdl.handle.net/10498/29686
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026