RT preprint
T1 Yield evaluation of enzyme hydrolysis and dark fermentation of the brown seaweed Rugulopteryx okamurae hydrothermally pretreated by microwave irradiation
A1 Fernández Medina, Pedro
A1 Álvarez Gallego, Carlos José
A1 Caro Pina, Ildefonso
A2 Ingeniería Química y Tecnología de Alimentos
K1 Microwave pretreatment
K1 Hydrothermal pretreatment
K1 dark fermentation
K1 Enzyme hydrolysis
K1 Brown seaweed
AB Microwave irradiation has been applied to solubilise the organic matter from the invasive seaweed Rugulopterix okamurae as pretreatment for two conventional biological processes: enzyme hydrolysis (EH) for obtaining reducing sugars (RS) and dark fermentation (DF) for obtaining volatile fatty acids (VFAs). As operational conditions for the microwave pretreatment, temperature and time has been tested between 120 and 220 °C and 5–20 min respectively. The maximum solubilisation yield in terms of DOC (26%) through the microwave pretreatment was registered at 220 °C at 20 min. However, the maximum concentration for RS (1.8 g/L) was obtained at 200 °C at 20 min due to the decomposition of monomeric sugars at high temperature and the total polyphenols (TP) were above 0.25 g/L (where inhibition in dark fermentation begins to be noticiable) at 180 °C. When the pretreated biomass was used for the EH the maximum accumulated RS production was 160 mg-RS/g-biomass, at 220 °C which was a 35% better than the RS production for non-pretreated biomass. In the case of the DF tests, the maximum yield of VFAs obtained with the pretreated biomass was 46.3 mg/g-biomass at 220 °C while the maximum hydrogen yield was obtained at 200 °C (6.6 mL-H2/g-biomass) due to the high level of TP (1.1 g/L) of pretreated biomass at 220 °C. Despite of this toxic effect, the VFAs production at 220 °C was 93% higher than in the non-pretreated biomass.
PB Elsevier
SN 2213-2929
YR 2022
FD 2022-10-18
LK http://hdl.handle.net/10498/29759
UL http://hdl.handle.net/10498/29759
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026