RT journal article
T1 Feasibility of exhausted sugar beet pulp as raw material for lactic acid production
A1 Díaz Sánchez, Ana Belén
A1 Blandino Garrido, Ana María
A1 Caro Pina, Ildefonso
A1 Marzo Gago, Cristina
A1 González Pineda, Claudia
A2 Ingeniería Química y Tecnología de Alimentos
K1 Sugar beet pulp
K1 simultaneous saccharification and fermentation
K1 lactic acid
K1 pH control
K1 fed-batch SSF
AB Exhausted sugar beet pulp pellets (ESBPP), a sugar industry by-product generated after sugar extraction in the sugar production process, have been used as a raw material for lactic acid (LA) production via hydrolysis and fermentation by Lactobacillus casei. The present study proposes simultaneous saccharification and fermentation (SSF) of ESBPP to design a more cost-effective process. The effects of pH control, nutrient supplementation and solid addition in fed-batch SSF on lactic acid production were investigated.RESULTSThe highest LA concentration (26.88 g L–1) was reached in fed-batch SSF at a solid/liquid loading of 0.2 g mL–1, with pH control (by adding 30 g L–1 CaCO3 to the medium) and nutrient supplementation (by adding 20 mL of MRS medium per 100 mL of buffer). Under these conditions, a maximum productivity of 0.63 g L−1 h−1 was achieved, 2.7 times higher than that of the control experiment (SSF inoculated at time 0 h). However, a slightly lower LA yield was obtained, revealing the need for an increasing dose of enzymes at high solid-loading SSF.CONCLUSIONAn efficient fed-batch SSF strategy with pH control and MRS supplementation is described in the present study, attaining higher LA productivity compared to separate hydrolysis and fermentation and SSF.
PB Wiley Online Library
YR 2020
FD 2020-02-14
LK http://hdl.handle.net/10498/29780
UL http://hdl.handle.net/10498/29780
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026