RT journal article T1 Rugulopteryx okamurae: Effect of hydrothermal acid pretreatment on the saccharification process A1 Romero Vargas, Agustín A1 Fernández Güelfo, Luis Alberto A1 Blandino Garrido, Ana María A1 Díaz Villanueva, Manuel Jesús A1 Díaz Sánchez, Ana Belén A2 Ingeniería Química y Tecnología de Alimentos K1 Macroalga K1 Dietary fibre K1 Enzyme hydrolysis K1 Combined severity factor K1 Reducing sugar AB The biological invasion caused by the invasive macroalga Rugulopteryx okamurae is causing increasing concern in southern Europe. To reduce its impact, this brown alga can be treated from a biorefinery approach. In this study, the macroalga is used as raw material to obtain fermentable sugars, which can be converted into high-value-added products. The alga was exposed to hydrothermal and hydrothermal acid pretreatment and the pretreated biomass was used for enzymatic hydrolysis, achieving a hydrolysate with a reducing sugar concentration of almost 25 g/L (49.2% more than with non-pretreated alga). In addition, the combined severity factor was calculated to identify the best pretreatment conditions, finding the optimum in those pretreatments performed with 0.2 N HCl concentration and 15 min reaction time. Based on the results, it would be interesting to carry out new studies using the saccharified medium obtained under optimal conditions to obtain value-added compounds by fermentation. PB Elsevier YR 2023 FD 2023-11 LK http://hdl.handle.net/10498/29783 UL http://hdl.handle.net/10498/29783 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 11-nov-2026