RT journal article
T1 Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients
A1 Beltrán Camacho, Lucía
A1 Bhosale, S.D.
A1 Sánchez Morillo, Daniel
A1 Sánchez Gomar, Ismael
A1 Rojas Torres, Marta
A1 Eslava Alcón, Sara
A1 Martínez-Torija, Mario
A1 Ruiz de Infante, Mª Angeles
A1 Nieto-Martín, Mª Dolores
A1 Rodríguez Iglesias, Manuel Antonio
A1 Moreno, Juan A.
A1 Berrocoso Domínguez, Esther María
A1 Larsen, Martin R.
A1 Moreno-Luna, Rafael
A1 Durán Ruiz, María del Carmen
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 BiomedicinaBiotecnología y Salud Pública
A2 Ingeniería en AutomáticaElectrónica, Arquitectura y Redes de Computadores
A2 Neurociencias
K1 COVID-19
K1 ECFCs
K1 Viral infection
K1 RNA metabolism
K1 cardiovascular disease
K1 endothelial dysfunction
K1 autophagy
K1 proteomics
K1 mass spectrometry
AB Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection significantly affects the cardiovascular system, causing vascular damage and thromboembolic events in critical patients. Endothelial dysfunction represents one of the first steps in response to COVID-19 that might lead to cardiovascular complications and long-term sequelae. However, despite the enormous efforts in the last two years, the molecular mechanisms involved in such processes remain poorly understood. Herein, we analyzed the protein changes taking place in endothelial colony forming cells (ECFCs) after the incubation with the serum from individuals infected with COVID-19, whether asymptomatic or critical patients, by application of a label free-quantitative proteomics approach. Specifically, ECFCs from healthy individuals were incubated ex-vivo with the serum of either COVID-19 negative donors (PCR-/IgG-, n:8), COVID-19 asymptomatic donors at different infective stages (PCR+/ IgG-, n:8and PCR-/IgG+, n:8), or hospitalized critical COVID-19 patients (n:8), followed by proteomics analysis. In total, 590 proteins were differentially expressed in ECFCs in response to all infected serums. Predictive analysis highlighted several proteins like CAPN5, SURF4, LAMP2 or MT-ND1, as highly discriminating features between the groups compared. Protein changes correlated with viral infection, RNA metabolism or autophagy, among others. Remarkably, the angiogenic potential of ECFCs in response to the infected serums was impaired, and many of the protein alterations in response to the serum of critical patients were associated with cardiovascular-related pathologies.
PB IVYSPRING INT PUBL
SN 1449-2288
YR 2023
FD 2023-05-03
LK http://hdl.handle.net/10498/29938
UL http://hdl.handle.net/10498/29938
LA eng
NO This study was supported by GLOBALCAJA-Ayuda COVID-19; and Fondo Supera COVID-19, funded by Banco Santander and CRUE universidades, Ref. IPSA-COVID-19, and the Institute of Health Carlos III, ISCIII (PI18-00427, PI20-00716), co-funded by European Regional Development “A way to make Europe”.
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026