RT journal article
T1 Rugulopteryx-derived spatane, secospatane, prenylcubebane and prenylkelsoane diterpenoids as inhibitors of nitric oxide production
A1 Cuevas, Belén
A1 Arroba Espinosa, Ana Isabel
A1 Reyes Jiménez, Carolina de los
A1 Zubía Mendoza, Eva
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Orgánica
K1 diterpenoids
K1 brown algae
K1 Rugulopteryx okamurae
K1 anti-inflammatory
K1 NO inhibition
AB This study aimed to evaluate the anti-inflammatory potential of the different classesof diterpenoids produced by algae of the genus Rugulopteryx. First, sixteen diterpenoids (1–16),including spatane, secospatane, prenylcubebane, and prenylkelsoane metabolites, were isolated fromthe extract of the alga Rugulopteryx okamurae collected at the southwestern Spanish coasts. Eight ofthe isolated diterpenoids are new compounds whose structures were determined by spectroscopicmeans: the spatanes okaspatols A-D (1–4); the secospatane rugukamural D (8); the prenylcubebanesokacubols A (13) and B (14); and okamurol A (16), which exhibits an unusual diterpenoid skeletonfeaturing a kelsoane-type tricyclic nucleus. Second, anti-inflammatory assays were performed onmicroglial cells Bv.2 and macrophage cells RAW 264.7. Compounds 1, 3, 6, 12, and 16 causedsignificant inhibition of the NO overproduction induced by LPS in Bv.2 cells, and compounds 3, 5, 12,14, and 16 significantly decreased levels of NO in LPS-stimulated RAW264.7 cells. The most activecompound was okaspatol C (3), which completely suppressed the effects of LPS stimulation, both inBv.2 and in RAW264.7 cells.
PB MDPI
SN 1660-3397
YR 2023
FD 2023-04-19
LK http://hdl.handle.net/10498/29960
UL http://hdl.handle.net/10498/29960
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026