RT journal article T1 Effect of gas-liquid flow pattern and microbial diversity analysis of a pilot-scale biotrickling filter for anoxic biogas desulfurization A1 Almengló Cordero, Fernando A1 Bezerra, T. A1 Lafuente, Javier A1 Gabriel, D. A1 Ramírez Muñoz, Martín A1 Cantero Moreno, Domingo A2 Ingeniería Química y Tecnología de Alimentos K1 Biotrickling filter K1 Anoxic K1 biogas desulfurization K1 Hydrogen sulfide K1 Pyrosequencing AB Hydrogen sulfide removal from biogas was studied under anoxic conditions in a pilot-scale biotrickling filter operated under counter- and co-current gas-liquid flow patterns. The best performance was found under counter-current conditions (maximum elimination capacity of 140 gS m−3 h−1). Nevertheless, switching conditions between co- and counter-current flow lead to a favorable redistribution of biomass and elemental sulfur along the bed height. Moreover, elemental sulfur was oxidized to sulfate when the feeding biogas was disconnected and the supply of nitrate (electron acceptor) was maintained. Removal of elemental sulfur was important to prevent clogging in the packed bed and, thereby, to increase the lifespan of the packed bed between maintenance episodes. The larger elemental sulfur removal rate during shutdowns was 59.1 gS m−3 h−1. Tag-encoded FLX amplicon pyrosequencing was used to study the diversity of bacteria under co-current flow pattern with liquid recirculation and counter-current mode with a single-pass flow of the liquid phase. The main desulfurizing bacteria were Sedimenticola while significant role of heterotrophic, opportunistic species was envisaged. Remarkable differences between communities were found when a single-pass flow of industrial water was fed to the biotrickling filter. PB ELSEVIER SN 0045-6535 YR 2016 FD 2016-08 LK http://hdl.handle.net/10498/29988 UL http://hdl.handle.net/10498/29988 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 07-nov-2026