RT journal article
T1 Multiresponse optimization of a UPLC method for the simultaneous determination of tryptophan and 15 tryptophan-derived compounds using a Box-Behnken design with a desirability function
A1 Setyaningsih, Widiastuti
A1 Sapruto, Irfan E.
A1 Carrera Fernández, Ceferino Adrián
A1 Palma Lovillo, Miguel
A1 García Barroso, Carmelo
A2 Química Analítica
K1 Tryptophan
K1 Tryptophan derivatives
K1 Chromatographic separation
K1 Design of experiments
AB A Box–Behnken design was used in conjunction with multiresponse optimization based on the desirabilityfunction to carry out the simultaneous separation of tryptophan and 15 derivatives by Ultra Performance Liquid Chromatography. The gradient composition of the mobile phase and the flow rate were optimized with respect to the resolution of severely overlapping chromatographic peaks and the total run time. Two different stationary phases were evaluated (hybrid silica and a solid-core-based C18 column). The methods were validated and a suitable sensitivity was found for all compounds in the concentration range 1–100 lg L–1 (R2 > 0.999). High levels of repeatability and intermediate precision (CV less than 0.25% and 1.7% on average for the retention time and the signal area, respectively) were obtained. The new method was applied to the determination tryptophan and its derivatives in black pigmented glutinous and non- lutinous rice grain samples
PB Elsevier
SN 1873-7072
YR 2017
FD 2017
LK http://hdl.handle.net/10498/30064
UL http://hdl.handle.net/10498/30064
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026