RT journal article T1 Ultrasound-assisted extraction of anthocyanins and total phenolic compounds in Vitis vinifera L. 'Tempranillo' winemaking lees A1 Sancho Galán, Pau A1 Amores Arrocha, Antonio A1 Jiménez Cantizano, Ana Concepción A1 Ferreiro González, Marta A1 Palacios Macías, Víctor Manuel A1 Fernández Barbero, Gerardo A2 Ingeniería Química y Tecnología de Alimentos A2 Química Analítica K1 anthocyanins K1 winemaking lees K1 phenolic compounds K1 ultrasound-assisted extraction AB Winemaking lees (WL) are the less exploited by-products from the winemaking industry. This by-product is generated after the fermentation of grape must and its composition is variable, although it is mainly made up of lysed yeasts, tartaric acid, colloids, polyphenols and ashes. Of all the compounds that can be found in lees, polyphenols exhibit particularly powerful bioactive properties and their extraction for their reuse in different matrices could be a matter of particular interest.Ultrasound-assisted extraction (UAE) has been used to extract both total anthocyanins (TA) and total phenolic compounds (TPC) from lees from Vitis vinifera L. 'Tempranillo' vinification. The optimization of six extraction variables (% methanol, pH, temperature, amplitude, cycle and ratio solvent:mass) was performed using a Box-Behnken design (BBD) where TA and TPC were considered independent variables. The UAE method that has been developed in this research could be a new rapid and efficient method to extract TPC and, coupled to chromatographic methodologies, identify up to fourteen different anthocyanins from WL. The objective of this study is to determine WL polyphenolic content and its potential for further uses in other procedures aiming to create value from vinification waste. PB Julius Kühn-Institut Institute for Grapevine Breeding Geilweilerhof 76833 Siebeldingen (Germany) SN 2367-4156 YR 2019 FD 2019-12-13 LK http://hdl.handle.net/10498/30211 UL http://hdl.handle.net/10498/30211 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 11-nov-2026