RT journal article T1 Development of New Analytical Microwave-Assisted Extraction Methods for Bioactive Compounds from Myrtle (Myrtus communis L.) A1 Velasco González de Peredo, Ana A1 Vázquez Espinosa, Mercedes A1 Espada Bellido, Estrella A1 Jiménez Cantizano, Ana Concepción A1 Ferreiro González, Marta A1 Amores Arrocha, Antonio A1 Palma Lovillo, Miguel A1 García Barroso, Carmelo A1 Fernández Barbero, Gerardo A2 Ingeniería Química y Tecnología de Alimentos A2 Química Analítica K1 anthocyanins K1 bioactive compounds K1 Box–Behnken design K1 microwave-assisted extraction K1 myrtle K1 Myrtus communis K1 phenolic compounds AB The phenolic compounds and anthocyanins present in myrtle berries are responsible for its beneficial health properties. In the present study, a new, microwave-assisted extraction for the analysis of both phenolic compounds and anthocyanins from myrtle pulp has been developed. Different extraction variables, including methanol composition, pH, temperature, and sample–solvent ratio were optimized by applying a Box–Behnken design and response surface methodology. Methanol composition and pH were the most influential variables for the total phenolic compounds (58.20% of the solvent in water at pH 2), and methanol composition and temperature for anthocyanins (50.4% of solvent at 50 °C). The methods developed showed high repeatability and intermediate precision (RSD < 5%). Both methods were applied to myrtle berries collected in two different areas of the province of Cadiz (Spain). Hierarchical clustering analysis results show that the concentration of bioactive compounds in myrtle is related to their geographical origin. PB MDPI SN 1420-3049 YR 2018 FD 2018-11-16 LK http://hdl.handle.net/10498/30214 UL http://hdl.handle.net/10498/30214 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 11-nov-2026