RT journal article
T1 Preparation and characterization of fluorescent CdS quantum dots used for the direct detection of GsT fusion proteins
A1 Fernández Ponce, Cecilia Matilde
A1 Blanco Ollero, Eduardo
A1 Barrera-Solano, M. Carmen
A1 Ramírez del Solar, Milagrosa
A1 Domínguez de la Vega, Manuel
A1 García Cózar, Francisco José
A1 Litrán Ramos, Rocío
A2 BiomedicinaBiotecnología y Salud Pública
A2 Física de la Materia Condensada
K1 Quantum dots
K1 fluorescence
K1 glutathione
K1 glutathione S‐transferase fusion protein
AB Advances in the life sciences are now closely linked to the availability of new experimental tools that allow for the manipulation of biomolecules and the study of biological processes at the molecular level. In this context, we have optimized a synthesis process to obtain glutathione-capped fluorescent CdS nanoparticles to specifically detect Glutathione S-Transferase (GST) -tagged proteins. Using our method, based on five different heating steps, brightly fluorescent and biocompatible CdS quantum dots of different sizes can be obtained. QD optical behaviour has been evaluated studying both absorbance and fluorescence. For all the samples, the excitonic absorption onset clearly shows a blue shift at 512nm in comparison with that of bulk CdS, due to the quantum confinement effect. At increased average sizes of the nanocrystal, the emission fluorescent band shows a red shift, from 440nm to 540nm. Among different QD solutions, we demonstrate an expansion of the emission range up to ~100 nm, thus improving their features as biomarkers. Moreover we show that optimized glutathione-capped quantum dots can directly bind GST blotted onto polyvinylidene difluoride (PVDF) membranes, and thus are suitable for the direct detection of GST fusion proteins.
PB SAGE PUBLICATIONS LTD
SN 1847-9804
YR 2012
FD 2012-07-26
LK http://hdl.handle.net/10498/30288
UL http://hdl.handle.net/10498/30288
LA eng
NO Proyectos del Ministerio de Innovación y Ciencia (PN/PETRI/PR/2007‐019) y Junta de Andalucía (P08‐CTS‐04348)
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026