RT journal article
T1 Stress under the sun: Effects of exposure to low concentrations of UV-filter 4- methylbenzylidene camphor (4-MBC) in a marine bivalve filter feeder, the Manila clam Ruditapes philippinarum
A1 Santonocito, Melania
A1 Salerno, Barbara
A1 Trombini, Chiara
A1 Tonini, Federico
A1 Pintado Herrera, Marina Guadalupe
A1 Martínez-Rodríguez, Gonzalo
A1 Blasco, Julián
A1 Lara Martín, Pablo Antonio
A1 Hampel, Miriam
K1 4-methylbenzylidene camphor (4-MBC)
K1 Environmental levels
K1 Gene expression
K1 LC50
K1 Risk assessment
K1 Ruditapes philippinarum
K1 Survival
K1 UV filters
AB UV filters are a class of emerging contaminants with an annual estimated production of 10,000 tons worldwide that continuously enter aquatic environments. Among UV filters, 4-methylbenzylidenecamphor (4-MBC) is an organic camphor derivative used in the cosmetic industry for its ability to protect the skin against UV, specifically UV B radiation. Individuals of the Japanese clam, Ruditapes philippinarum, were exposed to 4-MBC at environmentally relevant and slightly higher concentrations (nominal: 0, 1, 10, 100 μg L−1) using a semi-static exposure system over a 7-days period followed by a 3-days depuration period (total 10 days) where no 4-MBC was added to the tanks. Assessed mortality reached up to 100 % at the highest exposure concentration and a LC50 value of 7.71 μg·L-14-MBC was derived. Environmental risk assessment carried out in a site specific environment, the Cadiz bay in the south of Spain, revealed a potential risk produced by the presence of 4-MBC. Digestive glands tissues were analysed for differential expression of genes encoding proteins involved in the stress response (SOD, MT, GST, EIF1, BCL2, TP53, CAT, 18S, GADPH, GPX, GADD45, THIO9) by RT-qPCR for relative quantification. Results showed that the presence of 4-MBC at environmentally relevant concentrations induced the expression of genes that encode for antioxidant enzymes (GST) and for proteins related to the inhibition of apoptosis (BCL2) and cellular stress (GADD), suggesting a physiological stress response
PB ELSEVIER
YR 2020
FD 2020-04
LK http://hdl.handle.net/10498/30323
UL http://hdl.handle.net/10498/30323
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026