RT journal article
T1 Ultrastructural Localization and Molecular Associations of HCV Capsid Protein in Jurkat T Cells.
A1 Fernández Ponce, Cecilia Matilde
A1 Durán Ruiz, María del Carmen
A1 Narbona Sánchez,  Isaac
A1 Muñoz Miranda, Juan Pedro
A1 Martínez de Arbulo Echevarría, Miguel
A1 Serna-Sanz, Antonio
A1 Baumann, Christian
A1 Litrán Ramos, Rocío
A1 Aguado Vidal, Enrique
A1 Bloch, Wilhelm
A1 García Cózar, Francisco José
A2 BiomedicinaBiotecnología y Salud Pública
K1 Hepatitis C virus
K1 immune evasion
K1 immune tolerance
K1 interactome
K1 proteomics
K1 regulatory T cells
K1 ultrastructure
AB Hepatitis C virus core protein is a highly basic viral protein that multimerizes with itself to form the viral capsid. When expressed in CD4+ T lymphocytes, it can induce modifications in several essential cellular and biological networks. To shed light on the mechanisms underlying the alterations caused by the viral protein, we have analyzed HCV-core subcellular localization and its associations with host proteins in Jurkat T cells. In order to investigate the intracellular localization of Hepatitis C virus core protein, we have used a lentiviral system to transduce Jurkat T cells and subsequently localize the protein using immunoelectron microscopy techniques. We found that in Jurkat T cells, Hepatitis C virus core protein mostly localizes in the nucleus and specifically in the nucleolus. In addition, we performed pull-down assays combined with Mass Spectrometry Analysis, to identify proteins that associate with Hepatitis C virus core in Jurkat T cells. We found proteins such as NOLC1, PP1γ, ILF3, and C1QBP implicated in localization and/or traffic to the nucleolus. HCV-core associated proteins are implicated in RNA processing and RNA virus infection as well as in functions previously shown to be altered in Hepatitis C virus core expressing CD4+ T cells, such as cell cycle delay, decreased proliferation, and induction of a regulatory phenotype. Thus, in the current work, we show the ultrastructural localization of Hepatitis C virus core and the first profile of HCV core associated proteins in T cells, and we discuss the functions and interconnections of these proteins in molecular networks where relevant biological modifications have been described upon the expression of Hepatitis C virus core protein. Thereby, the current work constitutes a necessary step toward understanding the mechanisms underlying HCV core mediated alterations that had been described in relevant biological processes in CD4+ T cells.
PB FRONTIERS MEDIA SA
SN 1664-302X
YR 2018
FD 2018-01-04
LK http://hdl.handle.net/10498/30374
UL http://hdl.handle.net/10498/30374
LA eng
NO Ministry of Education and Science, Spain [SAF2009-09449]; ISCIII [PI16/00784] from the “Plan Estatal de I+D+I 2013-2016/FEDER”; Consejería de Salud de la Junta de Andalucía, Spain [SAS 111206]
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026