RT journal article
T1 Effect of amino acid supplementation and stress on expression of molecular markers in meagre (Argyrosomus regius).
A1 Herrera, M.
A1 Matías, A.C.
A1 Soares, F.
A1 Ribeiro, L.
A1 Moreira, M.
A1 Salamanca, M.
A1 Jerez Cepa, Ismael
A1 Mancera Romero, Juan Miguel
A1 Astola González, Antonio
A2 Biología
A2 BiomedicinaBiotecnología y Salud Pública
K1 Stress
K1 Amino acid
K1 Meagre
K1 Molecular marker
AB The objectives of this work were: 1) develop of molecular stress biomarkers obtaining sequence data of differenttranscripts, 2) study the molecular stress response through the expression quantification of key gene involved init, and 3) assess the effects of dietary amino acid additives on stress response in meagre meagres (Argyrososmusregius). Fish batches were fed two experimental diets with tryptophan (Trp) or aspartate (Asp) added for sevendays. Before sampling fish were submitted to confinement/netting stress during 1 h, except control fish.Therefore fish were sampled before and after stress (1 h and 6 h post-stress). The sampling consisted of blood andtissues (brain, hypophysis and liver). Several gene expressions related to the stress response were measured inthose tissues, and the cloning of corticotropin-releasing hormone (crh), corticotropin-releasing hormone bindingprotein (crh-bp), and thyrotropin-releasing hormone (trh) has been reported in meagre for the first time. In fact,fish fed an additional Asp diet did not present any sl, prl and gh expression changes, as for the control group.Contrarily, the Trp diet altered the prl and gh expressions after stress. For crh and crh-bp expressions, no significantdifferences were detected within the Asp diet hence that amino acid improved the stress response.However, Asp feeding, but not Trp, enhanced pomc-a expression after stress. Hsp70 expression varied for everytreatment, including the control feeding, indicating a late response at 6 h post-stress sampling, where both Aspand Trp treatments increased these expressions significantly. Concluding, the response of molecular stressmarkers to amino acid enriched diets was diverse. The stressor did not change significantly the relativeexpression of most analyzed genes for control feeding groups, though the Asp supplemented diet was moreeffective for attenuating molecular markers than the Trp one.
PB Elsevier B.V.
SN 0044-8486
YR 2021
FD 2021-03-15
LK http://hdl.handle.net/10498/30499
UL http://hdl.handle.net/10498/30499
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026