RT journal article
T1 Fast analysis of capsaicinoids in Naga Jolokia extracts (Capsicum chinense) by  high-performance liquid chromatography using fused core columns
A1 Stipcovich, Tea
A1 Fernández Barbero, Gerardo
A1 Ferreiro González, Marta
A1 Palma Lovillo, Miguel
A1 García Barroso, Carmelo
A2 Química Analítica
K1 Capsaicinoids
K1 Capsicum chinense
K1 fused-core columns
K1 HPLC
K1 Naga Jolokia
K1 peppers
AB A rapid high-performance liquid chromatography method with a C18 reverse-phase fused-core column has been developed for the determination and quantification of the main capsaicinoids (nornordihydrocapsaicin, nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin and homodihydrocapsaicin) present in Naga Jolokia peppers. A fused-core Kinetex TM C18 column (50 × 2.1 mm i.d.; 2.6 μm) was used for the analysis. The chromatographic separation was obtained with a gradient method in which the mobile phase was water (0.1% acetic acid) as solvent A and acetonitrile(0.1% acetic acid) as solvent B. The separation of all compounds was achieved in less than 3 minutes with a total analysis time (sample-to-sample) of 10 minutes. The robustness of the method was evaluated. The method showed excellent repeatability and intermediate precision expressed as coefficient of variance of less than 2%. The developed method was employed for the quantification of the major capsaicinoids present in different peppers and commercial products containing chilli peppers.
PB Elsevier
SN 1873-7072
YR 2018
FD 2018
LK http://hdl.handle.net/10498/30507
UL http://hdl.handle.net/10498/30507
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026