RT journal article
T1 Optimizing an Enzymatic Extraction Method for the Flavonoids in Moringa (Moringa oleifera Lam.) Leaves Based on Experimental Designs Methodologies
A1 Polo Castellano, Curro
A1 Mateos Bernal, Rosa María
A1 Visiedo García, Francisco Manuel
A1 Palma Lovillo, Miguel
A1 Fernández Barbero, Gerardo
A1 Ferreiro González, Marta
A2 Anatomía y Embriología Humana
A2 BiomedicinaBiotecnología y Salud Pública
A2 Neurociencias
A2 Química Analítica
K1 Moringa oleifera Lam
K1 extraction method
K1 antioxidant power
K1 phenolic compounds
K1 flavonoids
K1 DPPH
AB Moringa oleifera Lam. is known to have significant antioxidant properties. Because of this, the development of an optimal extraction method is crucial to obtain pharmacological products based on the bioactive compounds produced by this tree. Through a Plackett–Burman and a Box–Behnken design, enzymatic extraction conditions (temperature, agitation, solvent pH and composition, sample-to-solvent ratio, enzyme-to-sample ratio and extraction time) have been optimized using normalized areas (UA/g) as response variable and relative mass (mg/g) as quantification variable. Extractions were performed in an incubator, where all the extraction conditions could be digitally controlled. Thus, 58.9 °C, 50 rpm, 4.0 pH, 32.5% EtOH, 0.2 g sample in 15 mL solvent and 106 U/g were established as the optimal extraction conditions for the extraction with a mix of pectinases coming from Aspergillus niger. Under these optimal conditions, two-minute extractions were performed and evaluated through a single factor design. The enzymatic extraction method demonstrated its suitability to produce extracts with good antioxidant power (antioxidant activity 4.664 ± 0.059 mg trolox equivalent/g sample and total phenolic compounds 6.245 ± 0.101 mg gallic acid equivalent/g sample). The method was also confirmed to have good repeatability (1.39%) and intermediate precision (2.37%) levels.
PB MDPI
SN 2076-3921
YR 2023
FD 2023
LK http://hdl.handle.net/10498/30509
UL http://hdl.handle.net/10498/30509
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026